Method of making and using microarrays of biological materials
First Claim
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1. A method of making an array of selected anti-ligands comprising:
- (i) providing a library of anti-ligand molecules displayed for binding with a ligand on the surface of a replicable unit;
(ii) providing a mixture of ligands (iii) exposing the library to the mixture whereby ligand/anti-ligand binding can take place;
(iv) isolating and amplifying the number of anti-ligands which bind ligands; and
(v) applying a preparation of the same anti-ligands, or a plurality of different anti-ligands, to a separate region of a substrate to form an array of separate anti-ligand-containing regions on a solid support.
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Abstract
The invention relates to methods of making arrays of anti-ligands for use in the analysis of ligands. Preferred ligands are proteins and preferred anti-ligands are antibodies, or antigen-binding variants or derivatives thereof. The microarrays of the invention provide a convenient means for analysing differential protein expression in cells.
12 Citations
23 Claims
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1. A method of making an array of selected anti-ligands comprising:
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(i) providing a library of anti-ligand molecules displayed for binding with a ligand on the surface of a replicable unit;
(ii) providing a mixture of ligands (iii) exposing the library to the mixture whereby ligand/anti-ligand binding can take place;
(iv) isolating and amplifying the number of anti-ligands which bind ligands; and
(v) applying a preparation of the same anti-ligands, or a plurality of different anti-ligands, to a separate region of a substrate to form an array of separate anti-ligand-containing regions on a solid support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method or use substantially as described herein, preferably with reference to one or more of the examples 1 to 3.
Specification