System and methods for dynamic range extension using variable length integration time sampling
First Claim
1. A method for dynamic range extension during the processing of a photo-detector acquired signal, the method comprising:
- acquiring a first signal component and a second signal component from a photo-detector wherein the first signal component comprises an integration of the photo-detector signal during a first time interval and wherein the second signal component comprises integration of the photo-detector signal during a second time interval wherein the second time interval is temporally proximal to and shorter than the first time interval such that the second signal component and the first signal component represent the acquired values of the photo-detector signal during a selected time period delineated by the first and second time intervals;
determining a scaling factor between the second signal component and the first signal component;
determining if the first signal component exceeds a selected dynamic range;
if the first signal component exceeds the dynamic range, scaling the second signal component by the scaling factor to approximate the first signal component; and
using the scaled second signal component to represent the value of the signal during the selected time period.
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Abstract
A photo-detector generated signal is measured as a sample set comprising a long signal and a short signal. The short signal is scaled to the value of the long signal if the long signal exceeds a dynamic range associated with the photo detector. In one embodiment, the short signal is obtained during a short time interval that is at the approximate middle of a long time interval such that the short and long intervals share a common median time value. Given such symmetry, an approximately linear signal yields a proportionality parameter between the long and short signals thereby allowing the short signal to be scaled. The proportionality parameter facilitates determination of an integration independent component of the photo detector signal that should be removed from the measured long and short signals before scaling. A plurality of sample sets can also be processed such that each sample set overlaps with its neighboring sample set, thereby increasing the effective number of sample sets.
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Citations
57 Claims
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1. A method for dynamic range extension during the processing of a photo-detector acquired signal, the method comprising:
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acquiring a first signal component and a second signal component from a photo-detector wherein the first signal component comprises an integration of the photo-detector signal during a first time interval and wherein the second signal component comprises integration of the photo-detector signal during a second time interval wherein the second time interval is temporally proximal to and shorter than the first time interval such that the second signal component and the first signal component represent the acquired values of the photo-detector signal during a selected time period delineated by the first and second time intervals;
determining a scaling factor between the second signal component and the first signal component;
determining if the first signal component exceeds a selected dynamic range;
if the first signal component exceeds the dynamic range, scaling the second signal component by the scaling factor to approximate the first signal component; and
using the scaled second signal component to represent the value of the signal during the selected time period. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for scaling of a signal generated by a photo-detector signal processor, the method comprising:
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determining a first signal value L and a second signal value S for a sample set wherein the first signal value corresponds to a signal acquired during a first interval and wherein the second signal value corresponds to a signal acquired during a second interval, wherein the second signal value is less than the first signal value and wherein the first signal exceeds a specified range;
determining a proportionality parameter K between the first signal value and the second signal value; and
scaling the second signal value to approximate what the first signal value would be beyond the specified range. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A method of sampling a photo-detector signal, the method comprising:
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performing a series of integrations of the photo-detector signal wherein the series comprises alternating long and short integration intervals; and
forming a plurality of overlapping sample sets wherein each sample set comprises integrations performed during at least one long interval to yield a first signal value and at least one short interval to yield a second signal value and wherein each sample set overlaps with its neighboring sample set by at least one of the long or short intervals. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40)
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41. A system for processing a photo-detector signal associated with a sequencing apparatus, comprising:
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a photo-detector that detects a labeled sample signal that is transformed into an electronic signal;
an electronic signal processor that acquires one or more sample sets associated with the electronic signal wherein each sample set comprises a first signal value L and a second signal value S wherein the first signal value corresponds to an integrated photo-detector signal acquired during a first interval and wherein the second signal value corresponds to an integrated photo-detector signal acquired during a second interval that is less than the first interval; and
wherein the signal processor is configured to determine a proportionality parameter K between the first signal value and the second signal value such that the second signal value can be scaled to the first signal value and wherein the processor outputs a processed signal representative of the sample set based on the first and second signal values. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
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Specification