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Detection of nucleic acid polymorphisms

  • US 20040072200A1
  • Filed: 11/25/2003
  • Published: 04/15/2004
  • Est. Priority Date: 11/13/2000
  • Status: Abandoned Application
First Claim
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1. A method for characterizing nucleic acid polymorphisms, including the steps:

  • (a) provision of a nucleic acid template to be investigated, (b) annealing of at least one starting primer onto the nucleic acid template, with the 3′

    end of the starting primer being located upstream of a nucleic acid polymorphism to be investigated, (c) extension of the starting primer with at least one fluorescence-labeled nucleotide and (d) detection of nucleotides incorporated into the starting primer by single-molecule determination.

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