Thermal cycler

• US 20040072334A1
• Filed: 10/15/2002
• Published: 04/15/2004
• Est. Priority Date: 10/15/2002
• Status: Abandoned Application

• Current Assignee: Sicpa Holding SA
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Wheeler, Elizabeth K., Dzenitis, John M., Benett, William J., Krulevitch, Peter, Visuri, Steve, Stratton, Paul L., Richards, James B.
• Application Number:
  US10/272,178
• Publication Number:
  US 20040072334A1
• Time in Patent Office: Days
• US Class Current: 435/286.1
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A thermalcycler for processing a sample holder includes a first thermalcycler body with a first face and a second thermalcycler body with a second face. A cavity for receiving the sample holder is formed in at least one of the first face or the second face. A thermalcycling unit is operatively connected to the cavity.

Claims

1. A thermalcycler for processing a sample holder, comprising:

a first thermalcycler body having a first face,

a second thermalcycler body having a second face,

a cavity in at least one of said first face or said second face for receiving said sample holder, and

a thermalcycling unit operatively connected to said cavity.

2. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body comprise a flexible circuit material.

3. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body comprise a circuit board material.

4. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body comprise an insulated flexible heating material.

5. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a portion made of a flexible circuit material.

6. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a portion made of a circuit board material.

7. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a portion made of an insulated flexible heating material.

8. The thermalcycler of claim 1, wherein said cavity is in a shape to receive a tubular sample holder.

9. The thermalcycler of claim 1, wherein said cavity is in a shape to receive a tubular flow through sample holder.

10. The thermalcycler of claim 1, wherein said cavity is in a shape to receive a batch sample holder.

11. The thermalcycler of claim 1, wherein said cavity is in a shape to receive a test tube sample holder.

12. The thermalcycler of claim 1, wherein said thermalcycling unit includes a heating device.

13. The thermalcycler of claim 1, wherein said thermalcycling unit includes a precision resistor.

14. The thermalcycler of claim 1, wherein said thermalcycling unit includes an RTD.

15. The thermalcycler of claim 1, wherein said thermalcycling unit includes an RTD that provides temperature sensing of said PCR unit.

16. The thermalcycler of claim 1, wherein said thermalcycling unit includes a fan which aids in rapid thermal cycling.

17. The thermalcycler of claim 1, wherein said thermalcycling unit includes a pneumatic air source which aids in rapid thermal cycling.

18. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a conductive section that contains said cavity.

19. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a conductive copper section that contains said cavity.

20. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section.

21. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section made of a flexible circuit material.

22. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section made of a circuit board material.

23. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section that supports a heating device.

24. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section that supports a resistor.

25. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section that supports a precision resistor.

26. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section that supports an RTD.

27. The thermalcycler of claim 1, wherein said cavity includes multiple individual cavities and said thermalcycling unit includes multiple individual thermalcycling devices in said individual cavities.

28. The thermalcycler of claim 1, including at least one window that allows optical interrogation and detection of said sample holder.

29. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a frame section that supports a pneumatic air source which aids in rapid thermal cycling.

30. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a conductive section that contains said cavity and a frame section and including a conductive pad section positioned between said conductive section and said frame section.

31. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a conductive section that contains said cavity and a frame section and including a conductive pad section made of a thermally conductive elastomer material positioned between said conductive section and said frame section.

32. The thermalcycler of claim 1, wherein said first thermalcycler body and said second thermalcycler body include a conductive section that contains said cavity, a frame section, and a conductive pad section positioned between said conductive section and said frame section and including an elastomer pad section positioned adjacent said frame section.

33. A method of constructing a thermalcycler, comprising the steps of:

constructing a first thermalcycler body section having a first face,

constructing a second thermalcycler body section having a second face,

forming a cavity in at least one of said first face or said second face,

positioning a thermalcycler unit operatively connected to said cavity, and

connecting said first thermalcycler body section and said second thermalcycler body section together wherein said first face and said face are opposed to each other.

34. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed of a flexible circuit material.

35. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed of a circuit board material.

36. The method of constructing a thermalcycler of claim 33, wherein said cavity is formed in a shape to receive a tubular sample holder.

37. The method of constructing a thermalcycler of claim 33, wherein said cavity is formed in a shape to receive a tubular flow through sample holder.

38. The method of constructing a thermalcycler of claim 33, wherein said cavity is formed in a shape to receive a batch sample holder.

39. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes a heating device.

40. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes a resistor.

41. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes a precision resistor.

42. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes an RTD.

43. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes an RTD that provides temperature sensing of said PCR unit.

44. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes a fan which aids in rapid thermal cycling.

45. The method of constructing a thermalcycler of claim 33, said thermalcycling unit includes a pneumatic air source which aids in rapid thermal cycling.

46. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section.

47. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section made of a flexible circuit material.

48. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section made of a circuit board material.

49. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with a heating device.

50. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with a resistor.

51. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with a precision resistor.

52. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with an RTD.

53. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with an RTD that provides temperature sensing of said PCR unit.

54. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with a fan which aids in rapid thermal cycling.

55. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section with a pneumatic air source which aids in rapid thermal cycling.

56. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section and a conductive section with a conductive pad section positioned between said conductive section and said frame section.

57. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section and a conductive section with a conductive pad section made of a conductive elastomer material positioned between said conductive section and said frame section.

58. The method of constructing a thermalcycler of claim 33, wherein said first thermalcycler body and said second thermalcycler body are constructed to include a frame section and a conductive section with an elastomer pad section positioned adjacent said frame section.