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Method for inducing differentiation of embryonic stem cells into functioning cells

  • US 20040072344A1
  • Filed: 07/25/2003
  • Published: 04/15/2004
  • Est. Priority Date: 01/25/2002
  • Status: Abandoned Application
First Claim
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1. A method for inducing differentiation of mammalian embryonic stem cells into functioning cells, which comprises the steps of:

  • 1) culturing the mammalian embryonic stem cells together with feeder cells with a medium comprising leukemia inhibitory factor;

    2) culturing the cells obtained from step

         1) in absence of feeder cells with a medium comprising leukemia Inhibitory factor and basic FGF in a suspension culture condition to give embryonic bodies;

    3) culturing the obtained embryonic bodies with a selection-expanding medium; and

    4) culturing the obtained cell clusters with a differentiation medium to give functioning cells.

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