Low dead volume extraction column device
First Claim
1. A low dead volume extraction column comprising:
- i) a column body having an open upper end for attachment to a pump, an open lower end for passing fluid into and out of the column body, and an open channel between the upper and lower end of the column body;
ii) a bottom frit bonded to and extending across the open channel, the bottom frit having a low pore volume;
iii) a top frit bonded to and extending across the open channel between the bottom frit and the open upper end of the column body, the top frit having a low pore volume, wherein the top frit, bottom frit, and channel surface define an extraction media chamber; and
iv) a bed of extraction media positioned inside the extraction media chamber.
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Abstract
The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.
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Citations
34 Claims
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1. A low dead volume extraction column comprising:
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i) a column body having an open upper end for attachment to a pump, an open lower end for passing fluid into and out of the column body, and an open channel between the upper and lower end of the column body;
ii) a bottom frit bonded to and extending across the open channel, the bottom frit having a low pore volume;
iii) a top frit bonded to and extending across the open channel between the bottom frit and the open upper end of the column body, the top frit having a low pore volume, wherein the top frit, bottom frit, and channel surface define an extraction media chamber; and
iv) a bed of extraction media positioned inside the extraction media chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification