Fluid-type multiple electrochemical system and preparation thereof

• US 20040084306A1
• Filed: 12/30/2002
• Published: 05/06/2004
• Est. Priority Date: 10/30/2002
• Status: Active Grant

• Current Assignee: Intellectual Discovery Co., Ltd.
• Original Assignee: Electronics and Telecommunications Research Institute
• Inventors: Kang, Sun Kil, Shin, Dong-Ho, Kim, Youn Tae, Kim, Hyokyum, Yang, Haesik
• Granted Patent: US 6,969,451 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: G01N 27/27 Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

Provided are a fluid-type multiple electrochemical system to be applied to a biosensor manufacturing process. The system performs the processes of forming an internal layer or an enzyme layer on an electrode array formed on a substrate collectively by using a fluid having a simple structure. The system includes: a substrate for an electric circuit, which includes a plurality of electrode parts formed at regular intervals, the electrode part having a reference electrode and an auxiliary electrode; a fluid-type substrate having a fluid injection part, a fluid ejection part and a plurality of fluid storages, the fluid storages being formed at the same interval as the electrode part of the substrate for an electric circuit, and connected with each other through fluid passages; and a sensor substrate having a plurality of unit sensors formed at the same interval as the electrode part of the substrate for an electric circuit, each unit sensor being composed of an electrode part, an electrode pad for supplying power voltage simultaneously, and an electrode wiring. The fluid-type multiple electrochemical system can solve the problems of the conventional system, such as consumption of electrolyte, protein contamination, and degrading reproducibility of sensors, effectively.

Claims

1. A fluid-type multiple electrochemical system, comprising:

a substrate for an electric circuit, which includes a plurality of electrode parts formed at regular intervals, the electrode part having a reference electrode and an auxiliary electrode;

a fluid-type substrate having a fluid injection part, a fluid ejection part and a plurality of fluid storages, the fluid storages being formed at the same interval as the electrode part of the substrate for an electric circuit, and connected with each other through fluid passages; and

a sensor substrate having a plurality of unit sensors formed at the same interval as the electrode part of the substrate for an electric circuit, each unit sensor being composed of an electrode part, an electrode pad for supplying power voltage simultaneously, and an electrode wiring.

2. The system as recited in claim 1, wherein the electrode part of the sensor substrate includes a reference electrode, an auxiliary electrode, and a working electrode, and a protective layer is deposited on top of the reference and the auxiliary electrodes among the three electrodes.

3. The system as recited in claim 2, wherein the protective layer is a hydrogel selected from a group consisting of polyvinyl alcohol (PVA), polyacrylamide (PAAM), poly N-vinyl pyrrolidone (PNVP), polyhydroxyethyl methacrylate (PHEMA), polyethylene glycol (PEG), polyethylene oxide (PEO), polyethylene glycol monomethyl ether (PEGME) and cellulose.

4. The system as recited in claim 1, wherein an additional reference electrode and auxiliary electrode are inserted on one side of the fluid-type substrate.

5. A method for preparing a fluid-type multiple electrochemical system having a substrate for an electric circuit, a fluid-type substrate and a sensor substrate, comprising the steps of:

a) forming a plurality of electrode parts having a reference electrode and an auxiliary electrode on the substrate for an electric circuit at regular intervals;

b) forming a fluid injection part and a fluid ejection part on one side of the fluid-type substrate, forming a plurality of fluid storages at the same interval as the electrode parts formed on the substrate for an electric circuit, and forming fluid passages for connecting the fluid storages with each other; and

c) forming a plurality of unit sensors on the sensor substrate at the same interval as the electrode parts formed on the substrate for an electric circuit, the unit sensor including an electrode part having a reference electrode, an auxiliary electrode and a working electrode, an electrode pad and an electrode wiring.

6. The method as recited in claim 5, wherein the electrode pads are connected with each other so that power or power voltage could be supplied to the electrodes on the substrate for an electric circuit and the sensor substrate, simultaneously.

7. The method as recited in claim 5, wherein the fluid retained in the fluid storages of the fluid-type substrate contacts the electrode parts of the sensor substrate to form a plurality of electrochemical systems so as to perform an electrochemical reaction, simultaneously.

8. The method as recited in claim 5, wherein a protective layer is formed on top of the reference electrodes and auxiliary electrodes of the sensor substrate.

9. The method as recited in claim 8, wherein the protective layer is a hydrogel selected from a group consisting of polyvinyl alcohol (PVA), polyacrylamide (PAAM), poly N-vinyl pyrrolidone (PNVP), polyhydroxyethyl methacrylate (PHEMA), polyethylene glycol (PEG), polyethylene oxide (PEO), polyethylene glycol monomethyl ether (PEGME) and cellulose.