Method and system for processing regions of interest for objects comprising biological material
First Claim
1. A computer-implemented method for automated processing of a physical object comprising biological material, the method comprising:
- retrieving information indicating at least one region of interest of the physical object comprising biological material; and
based at least on the information indicating the region of interest, performing an operation on a physical location within the region of interest of the physical object comprising biological material.
2 Assignments
0 Petitions
Accused Products
Abstract
A method and apparatus are disclosed for processing regions of interest for objects comprising biological material. A region of interest can be denoted for a physical object and information indicating the region of interest can be stored in a computer-readable medium for later retrieval. Subsequently, when the object is retrieved, the information indicating the region of interest can be used to generate information specifying a physical location within the region of interest. An operation can then be performed on the physical location within the region of interest. Reference pints within the object can assist in regeneration of the region of interest, and the reference points can be arranged in such a fashion that processing can take rotation of the object into account. The invention includes various features advantageous for constructing tissue microarrays.
203 Citations
72 Claims
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1. A computer-implemented method for automated processing of a physical object comprising biological material, the method comprising:
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retrieving information indicating at least one region of interest of the physical object comprising biological material; and
based at least on the information indicating the region of interest, performing an operation on a physical location within the region of interest of the physical object comprising biological material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 69, 70, 71)
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28. A computer-readable medium comprising computer-readable instructions for performing the following to process a physical object comprising biological material:
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retrieving information indicating at least one region of interest of the physical object comprising biological material; and
based at least on the information indicating the region of interest, performing an operation on a physical location within the region of interest of the physical object comprising biological material. - View Dependent Claims (72)
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29. A computer-implemented method for processing an observable feature comprising biological material in a physical object, the method comprising:
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capturing a first image depicting the observable feature comprising biological material;
via the first image, denoting at least one region of interest comprising the feature comprising biological material;
storing information indicating the region of interest;
retrieving the information indicating the region of interest;
capturing a second image, wherein the second image depicts an item of known location and the object;
based on the second image and the retrieved information indicating the region of interest, generating information to position the feature comprising biological material at a location appropriate for extracting material from the feature comprising biological material;
sending the information to an automated positioning device to position the feature comprising biological material at a location appropriate for extracting material from the feature; and
extracting material from the feature comprising biological material. - View Dependent Claims (30, 31)
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32. A computer-implemented method for processing a physical object comprising biological material, the method comprising:
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during a first session, capturing a first image representative of the physical object comprising biological material;
during the first session, designating one or more regions of interest for the object comprising biological material via the first captured image;
during the first session, storing information indicating the one or more regions of interest for the physical object comprising biological material;
during a second, subsequent session, retrieving the physical object comprising biological material;
during the second, subsequent session, retrieving the information indicating the one or more regions of interest for the physical object comprising biological material;
during the second, subsequent session, capturing a second image of the physical object comprising biological material;
during the second, subsequent session, based on the second captured image and the retrieved information indicating the one or more regions of interest for the physical object comprising biological material, performing an operation on one or more physical locations within the one or more regions of interest for the physical object comprising biological material. - View Dependent Claims (33, 34, 35)
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36. A computer-implemented method for processing regions of interest in a set of physical objects comprising biological material, the method comprising:
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denoting a plurality of regions of interest for the physical objects comprising biological material;
selecting a list of a subset of the plurality of regions of interest; and
for the regions of interest appearing on the list, performing the following;
automatically retrieving a physical object comprising biological material having the region of interest; and
automatically extracting material from the region of interest of the physical object comprising biological material. - View Dependent Claims (37, 38)
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39. A computer-implemented method for processing a physical object comprising biological material, wherein the physical object comprises a plurality of reference points indicated thereon, the method comprising:
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retrieving information indicating a region of interest on the physical object comprising biological material with respect to the reference points;
capturing an image representing the physical object comprising biological material, the object'"'"'s reference points, and one or more system reference points;
finding locations of the object'"'"'s reference points and the system reference points on the image;
calculating a translation mapping location on the image to absolute locations sufficient to position a robotic arm at a physical location of the physical object corresponding to the locations on the image;
choosing a location within the region of interest;
with the translation, mapping the chosen location to physical location information sufficient to position an automated device at a physical location corresponding to the chosen location;
sending the physical location information to position the automated device at the physical location corresponding to the chosen location; and
with the automated device, performing an operation on the physical location within the region of interest of the physical object comprising biological material.
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40. A computer-implemented method for denoting one or more regions of interest for a physical object comprising biological material, wherein the physical object comprises a plurality of reference points, the method comprising:
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capturing an image representative of the physical object comprising biological material, wherein the image depicts locations of at least one of the reference points;
via the captured image, denoting one or more regions of interest for the physical object comprising biological material; and
in a computer-readable medium, storing information indicating the one or more regions of interest with respect to the reference points. - View Dependent Claims (41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. A computer-implemented method of translocating tissue from a donor tissue block to a recipient block, the method comprising:
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capturing a first image of a slice taken from the donor tissue block;
based on the first image, storing information indicative of a region of interest for the donor tissue block;
capturing an image of the donor tissue block;
based on the second image and the information indicative of a region of interest, regenerating the region of interest for the donor tissue block; and
via automated means, directing a tissue punch to a location appropriate for punching tissue from the region of interest of the donor tissue block;
with the tissue punch, punching tissue from the region of interest of the donor tissue block;
via automated means, directing the tissue punch to a location appropriate for depositing the tissue into the recipient block; and
with the tissue punch, depositing the tissue from the region of interest of the donor tissue block into the recipient block.
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57. A computer-readable medium comprising a data structure indicating a region of interest on a physical object comprising biological material, the data structure comprising:
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information indicating locations of a plurality of reference points on the physical object comprising biological material; and
information indicating a location and extent of a region of interest with respect to the reference points;
whereby the data structure, when processed by an automated system, causes regeneration of the region of interest. - View Dependent Claims (58, 59)
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60. A computer-implemented method for processing a plurality of tissue types, the method comprising:
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for a plurality of donor tissue blocks, denoting regions of interest on captured images of the donor tissue blocks, wherein the regions of interest comprise tissue of interest of a particular tissue type;
submitting a list of a subset of the regions of interest to an automated arrayer, wherein the list represents a plurality of tissue types; and
with the automated arrayer, based on the list, extracting tissue from the regions of interest and collecting them into a recipient tissue block to produce a recipient tissue block having each tissue type represented on the list. - View Dependent Claims (61, 62, 63, 64)
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65. An automated tissue microarray construction system comprising:
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an image capturing device operable to capture an image;
an automated tissue block retriever operable to retrieve one of a plurality of tissue blocks; and
a computer system operable to receive a captured image from the image capturing device;
wherein the computer system is further operable to accept a list of regions of interest from which material is to be extracted, retrieve the tissue blocks corresponding to the regions of interest, capture images of the retrieved blocks, and based on the images, extract tissue from the regions of interest appearing on the list. - View Dependent Claims (66)
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67. An automated tissue microarray construction system comprising:
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automated means for accepting designation of one or more regions of interest for a plurality of donor objects comprising tissue to be arrayed on one or more recipient objects; and
automated means for retrieving tissue from within the regions of interest of a plurality of donor objects and placing the tissue within one of the recipient objects. - View Dependent Claims (68)
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Specification