Microfluidic system for analyzing nucleic acids
First Claim
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1. A method of analyzing a nucleic acid target in a nucleic acid mixture of the target and non-target nucleic acids, the method comprising:
- attracting the nucleic acid mixture to an electrode included in electronics formed on a substrate;
retaining the target selectively by binding the target to a receptor disposed near the electrode;
enriching the mixture for the target by removing unretained nucleic acids; and
amplifying the target from the enriched mixture.
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Abstract
A system, including methods and apparatus, for microfluidic analysis of a nucleic acid target in a nucleic acid mixture. The system includes a method to preselect the target from the mixture before amplification. Preselection enriches the mixture for the target by retaining the target on a target-selective receptor and then removing unretained non-target nucleic acids. The preselected target then may be amplified from the enriched mixture and assayed. Devices configured to carry out the method are also disclosed.
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Citations
37 Claims
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1. A method of analyzing a nucleic acid target in a nucleic acid mixture of the target and non-target nucleic acids, the method comprising:
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attracting the nucleic acid mixture to an electrode included in electronics formed on a substrate;
retaining the target selectively by binding the target to a receptor disposed near the electrode;
enriching the mixture for the target by removing unretained nucleic acids; and
amplifying the target from the enriched mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A microfluidic device for analyzing a nucleic acid target in a nucleic acid mixture that includes the target, comprising:
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a substrate portion at least partially defining a chamber, the substrate portion including a substrate and electronics formed on the substrate, the electronics including at least first and second electrodes, each electrode being operable to form an electric field in the chamber; and
first and second receptors for specifically binding the target, the first and second receptors being distinct and connected to the first and second electrodes, respectively. - View Dependent Claims (17, 18, 19, 20, 21)
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22. A microfluidic device for analyzing a nucleic acid target in a nucleic acid mixture of the target and non-target nucleic acids, comprising:
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a substrate portion at least partially defining fluidically connected first and second chambers, the substrate portion including a substrate and electronics formed on the substrate, the electronics including a first electrode operable to form an electric field in the first chamber and a second electrode operable to form an electric field in the second chamber; and
first and second receptors for specifically binding the target, the first and second receptors being connected to the first and second electrodes, respectively. - View Dependent Claims (23, 24, 25, 26, 27)
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28. A microfluidic device for analyzing a nucleic acid target in a nucleic acid mixture of the target and non-target nucleic acids, comprising:
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a substrate portion at least partially defining a chamber, the portion including a substrate and electronics formed on the substrate, the electronics including an electrode configured to attract the nucleic acid mixture in the chamber;
a receptor connected to the electrode and configured to selectively bind the target from the attracted mixture; and
a fluid-handling portion connected to the substrate portion and configured to remove unbound non-target nucleic acids from the chamber and to deliver amplification reagents to the chamber. - View Dependent Claims (29, 30, 31)
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32. A microfluidic device for analyzing a nucleic acid target in a nucleic acid mixture of the target and non-target nucleic acids, comprising:
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means for attracting the nucleic acid mixture, the attracting means including an electrode included in electronics formed on a substrate;
means for selectively retaining the target near the attracting means;
means for removing unretained nucleic acids to enrich the mixture for the target; and
means for thermally cycling the enriched mixture to amplify the target from the enriched mixture. - View Dependent Claims (33, 34, 35, 36)
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37. A microfluidic device for analyzing a nucleic acid target in a nucleic acid mixture, comprising:
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means for preselecting the target from the mixture;
means for amplifying the preselected target; and
means for assaying the preselected target after amplification.
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Specification