Devices and methods for holding a biopolymeric array

• US 20040086874A1
• Filed: 10/31/2002
• Published: 05/06/2004
• Est. Priority Date: 10/31/2002
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Parker, Russell A.
• Application Number:
  US10/286,649
• Publication Number:
  US 20040086874A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/10 Means to control humidity and/or other gases
• CPC Class Codes: B01L 2300/105 using desiccants
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips

Abstract

Devices and methods for holding at least one array are provided. The subject devices are characterized by having a housing having at least one array therein and an absorbing material associated with the housing that is capable of absorbing molecules within the housing deleterious to the array(s) held therein. The seal may be resealable and/or may be a hermetic seal. The subject methods include packaging at least one array in a subject array holding device. Also provided are methods for using an array that is held in a subject array holding device in an array assay. Kits for use in the subject methods are also provided.

Claims

1. A device for holding at least one array therein, said device comprising:

(a) a housing having at least one array therein; and

(b) an absorbing material associated with said housing, whereby said absorbing material is capable of absorbing molecules within said housing deleterious to said at least one array.

2. The device according to claim 1, wherein said absorbing material is comprised of a polymer and an absorbing agent.

3. The device according to claim 1, wherein said polymer is chosen from the group of:

polypropylenes, polyethylenes, polyisoprenes, polybutadienes, polybutenes, polysiloxanes, polycarbonates, polyamides, ethylene-vinyl acetate copolymers, ethylene-methacrylate copolymers, poly(vinyl chloride), polystyrenes, polyesters, polyanhydrides, polyacrylonitriles, polysulfones, polyacrylic esters, acrylics, polyurethanes and polyacetals, polypropylene maleic anhydrides, polyethylene maleic anhydrides, polystyrene maleic anhydrides, polyethylene acrylic acids, polyethylene-uretheres, polyethylene-EVOH, polyethylene-nylons, grafted polyolefins, polyamides, ethylene-vinyl acetate partially hydrolyzed polymers, ethylene-methacrylate partially hydrolyzed polymers, grafted polyvinyl chlorides, grafted polystyrenes, polyesters, polyester amides, polyacrylic partially hydrolyzed esters, acrylics, polyurethanes, polyacetals, adhesives.

4. The device according to claim 1, wherein said absorbing agent is capable of absorbing at least one of:

water, acids, bases, corrosives, oxidizing gases, free radicals and reducing gases.

5. The device according to claim 1, wherein said absorbing agent is capable of absorbing at least one of:

inorganic chlorides, inorganic fluorides, sulfur compounds, nitrogen compounds, ozone, and organic compounds.

6. The device according to claim 2, wherein said absorbing agent is chosen from the group of metals, metal alloys, metal-plated particulates, inorganic molecules, molecular traps, surface-active materials, elastomers, semi-metals and ceramics.

7. The device according to claim 2, wherein said absorbing agent is chosen from the group of powdered iron, nickel, copper, aluminum, silicon, solder, silver, gold, silver-plated copper, silver-plated nickel, silver-plated glass microspheres, BaTiO₃, SrTiO₃, SiO₂, Al₂O₃, ZnO, TiO₂, MnO, CuO, Sb₂O₃ WC, alkali carbonates, alkali earth carbonates, fused silica, fumed silica, amorphous fuse silica, sol-gel silica, sol-gel titanates, mixed titanates, ion exchange resins, lithium-containing ceramics, hollow glass microspheres, zeolites, silica gels, clays, starches, carbon-based materials, carbon, activated charcoal, carbon black, ketchem black, diamond powder, fullerenes, derivatized nanotubes, polybutadienes, polysiloxanes, and calcium oxides.

8. The device according to claim 1, wherein said device comprises channels.

9. The device according to claim 8, wherein said channels are formed by at least one of a channeling agent, sintering, drilling, microcracking and laser cutting.

10. The device according to claim 9, wherein said channeling agent is hydrophilic.

11. The device according to claim 9, wherein said channeling agent is chosen from the group of polyethylene glycols, polypropylene glycols, mixtures of polyethylene glycols and polypropylene glycols, EVOH, glycerin, pentaerithritols, PVOH, polyvinylpyrollidines, vinylpyrollidone, N-methyl pyrollidones, and polysaccharides.

12. The device according to claim 1, wherein said housing is capable of forming a hermetic seal.

13. The device according to claim 12, wherein said seal is resealable.

14. The device according to claim 1, further comprising an emitting material associated with said housing that is capable of emitting molecules advantageous to said at least one array.

15. The device according to claim 14, wherein said emitting material is chosen from the group of antioxidants, anti-reducing agents, anti-acid agents and anti-radical agents.

16. The device according to claim 1, wherein said at least one array is an array of polymers.

17. The device according to claim 16, wherein said polymers are nucleic acids.

18. The device according to claim 16, wherein said polymers are peptides.

19. The device according to claim 1, wherein said absorbing material is in the form of a sheet, strip, film, layer, coating, laminate or plug.

20. The device according to claim 1, wherein said absorbing material is integral to said housing.

21. The device according to claim 20, wherein said absorbing material is co-molded with said housing.

23. The device according to claim 1, wherein said absorbing material is an insertable sleeve.

28. A method of performing an array assay, said method comprising:

(a) providing an array holding device according to claim 1;

(b) sealing said at least one array in said array holding device, whereby said absorbing materials absorbs molecules within said housing deleterious to said at least one array;

(c) breaking said seal and removing said at least one array from said device; and

(d) contacting said removed at least one array with sample to perform an array assay with said removed at least one array.

29. The method according to claims 28, further comprising reading said at least one array to obtain a result.

30. The method according to claim 29, further comprising forwarding data representing a result of said reading from a first location to a second location.

31. The method according to claim 30, wherein said second location is remote from said first location.

32. The method according to claim 29, further comprising receiving data representing a result of said reading.

33. The method according to claim 28, further comprising, after performing said array assay, resealing said at least one array in the same or different array holder and at some time thereafter breaking said seal and removing said resealed at least one array from said device.

34. The method according to claim 28, wherein said array holding device comprises more than one array and said method further comprises repeating steps B through C one or more times for any remaining arrays therein.

35. A kit for performing an array assay, said kit comprising:

(a) at least one device according to claim 1,

(b) instructions for using said at least one array in an array assay.

22. The device according to Clam 1, wherein said absorbing material forms said housing.

24. A method of packaging at least one array, said method comprising:

(a) providing an array holding device, said array holding device comprising;

(i) a housing having at least one array therein, and

(ii) an absorbing material associated with said housing, whereby said absorbing material is capable of absorbing molecules within said housing deleterious to said at least one array;

(b) positioning at least one array inside said array holding device; and

(c) sealing said at least one array inside said array holder, whereby said absorbing material absorbs molecules deleterious to said at least one array.

25. The method according to claim 24, wherein said seal is a hermetic seal.

26. The method according to claim 24, wherein said housing further comprises an emitting agent capable of emitting molecules within said housing that are beneficial to said at least one array.

27. The method according to claim 24, further comprising breaking and resealing said seal.