Composite flexible array substrate having flexible support

• US 20040087008A1
• Filed: 10/31/2002
• Published: 05/06/2004
• Est. Priority Date: 10/31/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Schembri, Carol T.
• Granted Patent: US 7,422,911 B2
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00518 in the shape of tapes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 : Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 : Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 : Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 : Laminated structure
• CPC Class Codes: B01L 2300/168 : Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/505 : flexible containers not provided for above
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 977/902 : Specified use of nanostructure

Abstract

Flexible array substrates having a flexible support, a flexible base, a reflective layer, and a transparent layer, in that order, are taught. Methods of forming the flexible array substrates, devices incorporating flexible array substrates, and arrays having probes arranged on a surface of the flexible array substrate are also taught.

Claims

1. A flexible array substrate comprising, in order,

a flexible support,

a flexible base,

a reflective layer, and

a transparent layer.

2. The flexible array substrate according to claim 1, wherein the optically transparent layer has surface functional groups adapted for having probes attached to said functional groups.

3. The flexible array substrate according to claim 1, wherein the reflective layer comprises a layer of a metal selected from aluminum, silver, gold, platinum, chrome, and tantalum.

4. The flexible array substrate according to claim 1, wherein the reflective layer comprises multiple dielectric layers.

5. The flexible array substrate according to claim 4, each dielectric layer being between about 40 nm and about 400 nm thick.

6. The flexible array substrate according to claim 1, wherein the transparent layer comprises silicon dioxide.

7. The flexible array substrate according to claim 1 wherein said flexible array substrate has surface functional groups adapted for binding probes thereto.

8. The flexible array substrate according to claim 1, wherein the flexible support provides a surface having an identifier.

9. The flexible array substrate according to claim 1, wherein the flexible support provides a surface for handling the flexible array substrate.

10. The flexible array substrate according to claim 1, wherein said flexible array substrate comprises a polymer material selected from the group consisting of polyacrylamide, polyetheretherketone, polyacrylate, polymethacrylate, polyesters, polyolefins, polyethylene, polytetrafluoro-ethylene, polypropylene, poly (4-methylbutene), polystyrene, poly(ethylene terephthalate), nitrocellulose, cellulose acetate, poly (vinyl chloride), polyamides, nylon, poly(vinyl butyrate), cross-linked dextran, and agarose.

11. The flexible array substrate according to claim 1 wherein said flexible array substrate comprises semi-crystalline polyetheretherketone.

12. The flexible array substrate according to claim 1 wherein the transparent layer supports an addressable collection of probes having a surface density of at least about 100 features per square centimeter.

13. A method of forming a flexible array, comprising

a) attaching a flexible base to a flexible support, and

b) depositing a plurality of different probes on the flexible base in an array format to form the flexible array.

14. The method according to claim 13, further comprising cutting the flexible base to a desired shape.

15. The method according to claim 13, wherein the flexible base has a reflective layer attached thereto.

16. The method according to claim 13, further comprising attaching a reflective layer to the flexible base.

17. The method according to claim 16, wherein attaching the reflective layer comprises depositing one or more layers of one or more materials selected from the group consisting of aluminum, silver, gold, platinum, chrome, tantalum, and a dielectric using a process selected from vacuum deposition, sputtering, evaporation, chemical vapor deposition, and plasma enhanced chemical vapor deposition.

18. The method according to claim 13, wherein the flexible base has a transparent layer attached thereto.

19. The method according to claim 13, further comprising attaching a transparent layer to the flexible base.

20. The method according to claim 13, wherein the plurality of different probes are deposited on the flexible base to form a flexible array having at least 100 features per square centimeter.