Method and apparatus for employing a tunable microfluidic device for optical switching, filtering and assaying of biological samples

• US 20040091392A1
• Filed: 08/08/2003
• Published: 05/13/2004
• Est. Priority Date: 08/09/2002
• Status: Abandoned Application

• Current Assignee: Sarnoff Corporation (SRI International, Inc.)
• Original Assignee: Sarnoff Corporation (SRI International, Inc.)
• Inventors: Zanzucchi, Peter John, McBride, Sterling Eduard
• Application Number:
  US10/638,111
• Publication Number:
  US 20040091392A1
• Time in Patent Office: Days
• US Class Current: 422/400
• CPC Class Codes: G01N 2021/391 Intracavity sample
• CPC Class Codes: G01N 21/39 using tunable lasers
• CPC Class Codes: G02B 6/12007 forming wavelength selective elements, e.g. multiplexer, demultiplexer
• CPC Class Codes: G02B 6/3536 involving evanescent coupling variation, e.g. by a moving element such as a membrane which changes the effective refractive index mode converters G02B6/14; adjustable lateral coupling between waveguides G02B6/283; electro- optical refractive index variations G02F1/0118
• CPC Class Codes: G02B 6/3538 based on displacement or deformation of a liquid controlling the intensity of light by displacement or deformation of a fluid in general G02B26/004; fluid-filled lens of variable focal length G02B3/14
• CPC Class Codes: G02B 6/355 1x2 switch, i.e. one input and a selectable single output of two possible outputs
• CPC Class Codes: G02B 6/357 Electrostatic force electrostatic forces controlling reflecting elements in general G02B26/0841

Abstract

In one embodiment, a tunable microfluidic device comprises at least one optical resonant cavity having a microfluidic channel disposed through a center thereof. At least one fluid is manipulated within the channel to change or “tune” an optical characteristic of the optical resonant cavity. In further embodiments, the tunable microfluidic device is incorporated into systems for assaying biological and/or chemical samples and for optical switching and/or filtering.

Claims

1. A tunable device comprising:

at least one input waveguide;

at least one cavity waveguide in communication with the at least one input waveguide;

at least one output waveguide in communication with the at least one cavity waveguide; and

at least one channel disposed through a center of the at least one cavity waveguide for carrying at least one fluid.

2. The tunable device of claim 1, wherein said at least one cavity waveguide comprises:

two or more cavity waveguides arranged in a series; and

wherein said at least one channel is disposed through the centers of each of the two or more cavity waveguides.

3. The tunable device of claim 2, wherein each of the two or more cavity waveguides has a different resonant property.

4. The tunable device of claim 1, wherein said at least one cavity waveguide comprises:

two or more cavity waveguides arranged in an array; and

wherein said at least one channel comprises two or more channels, wherein each of the two or more cavity waveguides has one of said channels disposed through its center.

5. The tunable device of claim 4, wherein each of the two or more cavity waveguides has a different resonant property.

6. The tunable device of claim 4, wherein said at least one fluid disposed within each of the two or more channels has different optical properties.

7. The tunable device of claim 1, further comprising:

at least one binder attached to an interior surface of at least one cavity waveguide.

8. The tunable device of claim 2, further comprising:

at least one binder attached to an interior surface of at least one of the two or more cavity waveguides.

9. The tunable device of claim 8, wherein said at least one binder comprises different binders that are attached to the interior surfaces of each of the two or more cavity waveguides.

10. The tunable device of claim 2, further comprising:

at least one binder attached to an interior surface of at least a portion of the channel located between two cavity waveguides.

11. The tunable device of claim 10, wherein said at least one binder comprises different binders that are attached to the interior surfaces of two or more portions of the channel.

12. The tunable device of claim 1, wherein said at least one fluid comprises two or more fluids that are contained within the at least one channel, where each of the two or more fluids has a different refractive index.

13. The tunable device of claim 1, wherein the at least one cavity waveguide has dimensions on the order of tens of micrometers.

14. The tunable device of claim 1, further comprising:

a dielectric force actuator for manipulating the at least one fluid contained within the at least one channel.

15. A method for tunable optical switching, comprising the steps of:

providing a signal to an input waveguide;

providing a plurality of cavity waveguides along said input waveguide, wherein each of said cavity waveguides has a channel disposed through a center of said cavity waveguide;

providing at least one output waveguide in communication with said plurality of cavity waveguides; and

passing at least one fluid through at least one of said channels to effect switching of said signal from said input waveguide to at least one output waveguide.

16. The method of claim 15, wherein said fluid disposed within each of said channels has different optical properties.

17. The method of claim 15, wherein said at least one fluid comprises two or more fluids having different optical properties that can be passed through each of said channels.

18. The method of claim 15, further comprising the step of:

providing a capillary break in at least one of said channels.

19. The method of claim 18, wherein the capillary break is disposed proximate to said cavity waveguide.

20. A method for assaying samples, the method comprising the steps of:

providing a signal to an input waveguide;

providing at least one cavity waveguide along said input waveguide, wherein each of said at least one cavity waveguides has a channel disposed through a center of said cavity waveguide;

providing at least one output waveguide in communication with said at least one cavity waveguide; and

passing at least one fluid through at least one of said channels to effect a change in the resonant properties of the at least one cavity waveguide, wherein the at least one fluid contains said sample.

21. The method of claim 20, wherein said fluid disposed within said at least one channel comprises two or more fluids that have different optical properties.

22. The method of claim 20, further comprising:

adding at least one assay reagent to said at least one fluid.

23. The method of claim 20, further comprising:

attaching a binder to an interior surface of at least one of said at least one cavity waveguides.

24. The method of claim 23, wherein the binder interacts with said sample in said at least one fluid.

25. The method of claim 20, further comprising:

attaching a binder to an interior surface of at least a portion of at least one of said channels, the portion residing between two cavity waveguides.

26. The method of claim 20, further comprising:

providing a capillary break in at least one of said channels.

27. The method of claim 26, wherein the capillary break is disposed proximate to said cavity waveguide.

28. A system for assaying samples, the system comprising:

an interface section, for introducing the samples into the system; and

a detection section, for analyzing one or more elements within the samples.

29. The system of claim 28, further comprising:

a deflection section, for sorting analyzed elements.

30. The system of claim 28, further comprising:

a focusing section for providing at least one reagent to the samples prior to analysis.

31. The system of claim 28, wherein the interface section comprises:

a sample reservoir for containing the samples, wherein the sample reservoir has an aperture;

a re-circulation channel in fluid communication with the sample reservoir for receiving said samples from the sample reservoir;

a detector positioned proximate to the re-circulation channel for separating elements out of the samples contained in the re-circulation channel; and

an interface channel in fluid communication with the re-circulation channel, for delivering the separated elements to a detection section.

32. The system of claim 31, wherein the re-circulation channel comprises:

an input portion for delivering a buffer solution for transporting the samples received from the sample reservoir; and

an output portion for delivering the buffer solution into the sample reservoir.

33. The system of claim 32, wherein the input and output portions of the recirculation channel are asymmetrically dimensioned relative to each other.

34. The system of claim 31, wherein the detector is at least one of an optical or electrical detector.

35. The system of claim 31, further comprising at least one electrode positioned proximate to the interface channel for creating an electric field.

36. The system of claim 30, wherein the focusing section comprises at least one transport channel for transporting said separated elements in a substantially single-file stream.

37. The system of claim 36, wherein the focusing section further comprises at least one lateral channel coupled to the transport channel for delivering said at least one reagent to the stream of separated elements.

38. The system of claim 36, wherein the separated biological elements are urged through the transport channel by at least one of electrohydrodynamic pumping, magnetohydrodynamic pumping, pressure pumping, electroosmotic pumping, electrophoresis, thermocapillarity and electrowetting.

39. The system of claim 28, wherein the detection section comprises a detector for analyzing the elements using at least one of light scattering, fluorescence spectroscopy, colorimetry, fluorescence polarization and surface plasmon resonance.

40. The system of claim 39, wherein the detector comprises at one least resonator assembly comprising:

at least one input waveguide;

at least one cavity waveguide in communication with the at least one input waveguide;

at least one output waveguide in communication with the at least one cavity waveguide; and

at least one transport channel disposed through a center of the at least one cavity waveguide for carrying said elements in at least one fluid.

41. The system of claim 40, further comprising:

at least one assay reagent disposed in said at least one transport channel.

42. The system of claim 40, wherein the dimensions of said at least one resonator assembly are on the order of tens of micrometers.

43. The system of claim 40, further comprising:

a binder attached to an interior surface of said at least one cavity waveguide.

44. The system of claim 40, further comprising:

two or more binders attached to interior surfaces of two or more cavity waveguides.

45. The system of claim 40, further comprising:

at least one binder attached to an interior surface of at least a portion of said transport channel, the portion residing between two cavity waveguides.

46. The system of claim 40, wherein said at least one cavity waveguide further comprises a dielectric force actuator for modifying the refractive index of said cavity waveguide.

47. Apparatus for interfacing a macrofluidic input source with a microfluidic system, comprising:

a reservoir for containing at least one input sample, wherein the reservoir has an aperture;

a re-circulation channel in fluid communication with the reservoir for receiving said at least one input sample from the reservoir;

a detector positioned proximate to the re-circulation channel for separating at least one element out of the at least one input sample contained in the recirculation channel; and

an interface channel in fluid communication with the re-circulation channel, for delivering at least one separated element to said microfluidic system.

48. Apparatus for sorting at least one element in a fluid-based sample, comprising:

at least one input channel;

two or more output channels coupled to the at least one input channel at a channel interface;

one or more electrodes positioned proximate to the channel interface for urging said at least one element into one of the two or more output channels.