Multiwell insert device that enables label free detection of cells and other objects
First Claim
1. A multiwell insert device, comprising:
- an upper chamber;
a lower chamber;
a membrane located between said upper chamber and said lower chamber; and
a sensor for detecting an object that passed through said membrane from said upper chamber into said lower chamber by measuring a change in a refractive index caused by the object being present on a surface of said lower chamber.
1 Assignment
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Accused Products
Abstract
A multiwell insert device and method for using the multiwell insert device are described herein. The multiwell insert device includes an upper chamber, a lower chamber, a membrane and a sensor for detecting in a label-free manner an object (e.g., cells, molecules, proteins, drugs, chemical compounds, nucleic acids, peptides, carbohydrates) that passed through the membrane from the upper chamber into the lower chamber by measuring a change in a refractive index caused by the object being present on a surface of the lower chamber. The multiwell insert device can be used to perform a wide-variety of assays including, for example, cell migration assays and drug permeability assays. In addition, the multiwell insert device can form or be incorporated into a well of a microplate.
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Citations
44 Claims
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1. A multiwell insert device, comprising:
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an upper chamber;
a lower chamber;
a membrane located between said upper chamber and said lower chamber; and
a sensor for detecting an object that passed through said membrane from said upper chamber into said lower chamber by measuring a change in a refractive index caused by the object being present on a surface of said lower chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for using a multiwell insert device, said method comprising the steps of:
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placing an object in an upper chamber of said multiwell insert device;
allowing the object to migrate from the upper chamber to a lower chamber through a membrane of said multiwell insert device; and
detecting the object on a surface of the lower chamber using a sensor that measures a change in a refractive index caused by the object being present on a surface of the lower chamber. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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37. A microplate, comprising:
a frame including a plurality of wells formed therein, each well is in the form of a multiwell insert device that includes;
an upper chamber;
a lower chamber;
a membrane located between said upper chamber and said lower chamber; and
a sensor for detecting an object that passed through said membrane from said upper chamber into said lower chamber by measuring a change in a refractive index caused by the object being present on a surface of said lower chamber. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44)
Specification