Porous silica substrates for polymer synthesis and assays
First Claim
1. A porous substrate comprising:
- a support region; and
a porous region on the support region, the porous region being primarily inorganic and having a surface capable of forming a polymer array thereon, the porous region comprising pores of a pore size of about 2 nm-500 nm, a porosity of about 10-90%, and a thickness of about 0.01 μ
m to about 70 μ
m.
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Abstract
Methods are provided for making and using thin films of porous silica substrates to synthesize arrays of polymers. Methods are also provided for assaying such polymers on porous silica substrates. The porous silica substrates offer an increase in array density and signal enhancement over conventional flat glass substrates. Examples of polymers that can be synthesized and assayed include biological polymers such as nucleic acids, polynucleotides, polypeptides, and polysaccharides. Arrays of nucleic acids or polynucleotides can be used for a variety of hybridization-based experiments such as nucleic acid sequence analysis, nucleic acid expression monitoring, nucleic acid mutation detection, speciation, effects of drug therapy on nucleic acid expression, among others.
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Citations
48 Claims
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1. A porous substrate comprising:
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a support region; and
a porous region on the support region, the porous region being primarily inorganic and having a surface capable of forming a polymer array thereon, the porous region comprising pores of a pore size of about 2 nm-500 nm, a porosity of about 10-90%, and a thickness of about 0.01 μ
m to about 70 μ
m. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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36. A porous substrate comprising:
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a support region; and
a porous region on the support region, said porous region of about 0.1-0.5 microns thick, wherein the porous layer comprises an unsintered matrix formed from at least colloidal silica having a particle size of about 70-100 microns, the unsintered matrix defining at least a plurality of open pores having a pore size of about 10-20 nm, and wherein the porous layer has a porosity of about 10-90%.
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37. A method of forming a porous substrate, the method comprising:
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providing a substrate material comprising a surface;
dipping the substrate material in a solution including colloidal silica and a carrier, the colloidal silica having a particle size of about 12-100 nm; and
withdrawing the substrate material to provide an unsintered porous layer having a thickness of about 0.1-1 microns and a porosity of about 10-90% on the substrate material.
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38. A method of forming a porous substrate, the method comprising:
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providing a substrate material comprising a surface;
applying a solution including colloidal silica and a carrier to the surface of the substrate material, the colloidal silica having a particle size of about 12-100 nm;
spinning the substrate material and the applied solution to achieve a spun layer on the substrate material; and
removing the carrier from the spun layer to provide an unsintered porous layer having a thickness of about 0.1-1 microns and a porosity of about 10-90% on the substrate material.
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Specification