Methods and compositions for encapsulating active agents
First Claim
1. A method of forming colloidosomes, comprising:
- (a) providing particles formed from a biocompatible material in a first solvent;
(b) forming an emulsion by adding a first fluid to said first solvent, said emulsion defined by droplets of said first fluid surrounded by said first solvent;
(c) coating the surface of said droplets with said particles; and
(d) stabilizing said particles on said surface of said droplet to form colloidosomes having a yield strength of at least about 20 Pascals.
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Abstract
Methods for making self-assembled, selectively permeable elastic microscopie structures, referred to herein as colloidosomes, that have controlled pore-size, porosity and advantageous mechanical properties are described. In one form of the invention, a method of forming colloidosomes includes providing particles formed from a biocompatible material in a first solvent and forming an emulsion by adding a first fluid to the first solvent wherein the emulsion is defined by droplets of the first fluid surrounded by the first solvent. The method includes coating the surface of droplet with the particles and the stabilizing the particles on the surface of droplet. The colloidosomes produced typically have a yield strength of at least about 20 Pascals. In certain forms of the invention, the particles are spherical and are formed of a biocompatible polymer. Colloidosomes formed according to the methods described herein are also provided. In one form, a colloidosome includes a shell formed of biocompatible, substantially spherical particles wherein each of the particles are linked to neighboring particles. The shell defines an inner chamber sized for housing a desired active agent and has a plurality of pores extending therethrough. The colloidosomes are structurally stable, typically having a yield strength of at least about 20 Pascals. Colloidal suspension and methods of encapsulating a desired active agent are also described.
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Citations
75 Claims
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1. A method of forming colloidosomes, comprising:
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(a) providing particles formed from a biocompatible material in a first solvent;
(b) forming an emulsion by adding a first fluid to said first solvent, said emulsion defined by droplets of said first fluid surrounded by said first solvent;
(c) coating the surface of said droplets with said particles; and
(d) stabilizing said particles on said surface of said droplet to form colloidosomes having a yield strength of at least about 20 Pascals. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of forming a colloidosome, comprising:
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(a) providing substantially spherical particles formed from a biocompatible polymer in a first solvent;
(b) forming an emulsion by adding a first fluid to said first solvent, said emulsion defined by droplets of said first fluid surrounded by said first solvent;
(c) coating the surface of said droplets with said spherical particles; and
(d) stabilizing said particles on said surface of said droplets to form colloidosomes having a yield strength of at least about 20 Pascals. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31)
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32. A method of encapsulating an active agent, comprising:
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(a) providing particles formed from a biocompatible material in a first solvent;
(b) forming an emulsion by adding a second solvent containing said active agent to said first solvent, said emulsion defined by droplets of said second solvent surrounded by said first solvent;
(c) coating the surface of said droplets with said particles; and
(d) stabilizing said particles on said surface of said droplet to form colloidosomes having a yield strength of at least about 20 Pascals. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. A method of encapsulating an active agent, comprising:
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(a) providing substantially spherical particles formed from a biocompatible polymer in a first solvent;
(b) forming an emulsion by adding a second solvent containing said active agent to said first solvent, said emulsion defined by droplets of said second solvent surrounded by said first solvent;
(c) coating the surface of said droplets with said spherical particles; and
(d) stabilizing said particles on said surface of said droplet to form a colloidosome. - View Dependent Claims (57, 58, 59, 60, 61, 62)
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63. A colloidosome, comprising:
a shell formed of biocompatible, substantially spherical particles, each of said particles linked to neighboring particles, said shell defining an inner chamber and having a plurality of pores extending therethrough, said chamber sized for housing an active agent, said colloidosome having a yield strength of at least about 20 Pascals. - View Dependent Claims (64, 65, 66, 67, 68, 69, 70, 71, 72, 73)
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74. A colloidosome suspension, comprising:
a colloidosome suspended in a first solvent, said colloidosome having a shell formed of biocompatible, substantially spherical particles, each of said particles linked to neighboring particles, said shell defining an inner chamber and having a plurality of pores extending therethrough, said chamber sized for housing an active agent and filled with a second solvent that is substantially similar to said first solvent. - View Dependent Claims (75)
Specification