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METHODS AND COMPOSITIONS FOR ENHANCING DETECTION IN DETERMINATIONS EMPLOYING CLEAVABLE ELECTROPHORETIC TAG REAGENTS

  • US 20040096825A1
  • Filed: 11/09/2001
  • Published: 05/20/2004
  • Est. Priority Date: 04/30/1999
  • Status: Active Grant
First Claim
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1. A method for isolating signal produced by a detectable moiety of a released predetermined portion of a reagent from signal obtained from the intact reagent and from other fragments thereof, the method comprising:

  • (a) combining (i) a first reagent comprising a releasable predetermined portion that includes a detectable moiety wherein the predetermined portion is attached to the first reagent by a cleavable moiety and wherein the first reagent comprises at least one interactive functionality adjacent the predetermined portion positioned in the first reagent such that the interactive functionality does not form part of the predetermined portion upon release thereof and (ii) a second reagent that potentiates the cleavage of the cleavable moiety, and (b) subjecting the combination to conditions under which the cleavable moiety is cleaved to release the predetermined portion, wherein the interactive functionality enables the isolation of the signal produced by the detectable moiety of the released predetermined portion from signal obtained from intact first reagent and from other fragments thereof.

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