Light-controlled electrokinetic assembly of particles near surfaces

• US 20040096846A1
• Filed: 03/10/2003
• Published: 05/20/2004
• Est. Priority Date: 04/25/1996
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Seul, Michael
• Granted Patent: US 7,291,504 B2
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00448 using microlens arrays
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: C40B 50/18 : using a particular method of attachment to the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Claims

1. A method for controlling the movement of particles suspended at an interface between an electrode and an electrolyte solution, the method comprising the following steps:

generating an electric field at said interface between said electrode and said electrolyte solution; and

illuminating the surface of said electrode with a predetermined light pattern to control the movement of said particles in accordance with said predetermined light pattern and electrochemical properties of said electrode.

2. The method of claim 1, wherein said electric field is at least one of a constant and a time varying electric field.

3. The method of claim 1, further comprising a patterning step which is performed using at least one of UV-mediated oxide regrowth, surface chemical patterning and surface charge profiling.

4. The method of claim 3, wherein said patterning step is used to create a plurality of areas of low impedance on said electrode, and said illuminating step is used to selectively connect one or more of said areas of low impedance to cause said particles to move therebetween in accordance with said patterning and said predetermined light pattern.

5. The method of claim 1, wherein the illuminating step comprises the further step of:

illuminating a selected area of said electrode which in conjunction with a frequency of said electric field causes the particles to move into said selected area.

6. The method of claim 5, wherein the frequency of said electric field is adjusted in order to move said particles out of said selected area.

7. The method of claim 1, wherein the illuminating step comprises the further step of:

illuminating a selected area of said electrode surface with a high intensity light pattern so as to cause the particles to move out of said selected area.

8. The method of claim 1 further comprising a patterning step which creates at least two areas of low impedance on said electrode, and said illuminating step being used to selectively cause said particles to move from a first low impedance area to a second low impedance area.

9. An apparatus for implementing the differential lateral displacement of particles suspended at an interface between an electrode and an electrolyte solution, said apparatus comprising:

an electric field generator which generates an electric field at said interface;

an electrode;

an electrolyte solution having a substantially continuous flow which effects the displacement of said particles in a direction substantially parallel to said interface;

an illumination source which illuminates said electrode with an adjustable, predetermined light pattern; and

a plurality of particles located in said electrolyte solution, said particles being in said electrolyte flow and being displaced by said electric field in conjunction with said predetermined light pattern, said particles being displaced in accordance with variations in physical and chemical properties of said particles which determine the mobility of said particles.

10. The apparatus of claim 9, wherein said electrode is a light sensitive electrode.

11. The apparatus of claim 9, wherein said impedance profile is created by a predetermined illumination pattern.

12. The apparatus of claim 9, wherein:

said electrode patterning includes an area of low impedance bordered by an area of high impedance, said low impedance area including, a narrow conduit in communication with a wide conduit, both said conduits being oriented parallel to the direction of said continuous flow of said electrolyte;

said wide conduit including a row of intermittently spaced areas of high impedance barriers traversing the width of said wide conduit;

a portion of said plurality of particles being optically distinguishable from the remaining particles;

a detector for visually inspecting said particles traversing the length of said narrow conduit in response to said continuous flow of electrolyte;

said illumination pattern being substantially in the shape of a rectangle having a longer dimension adjusted to be substantially equal to the width of said wide conduit, said rectangle having a smaller dimension which is adjusted to be substantially equivalent to the diameter of said particles, said pattern being located in front of said barriers, and said illumination pattern conforming to an intensity profile placing a maximal value of intensity in the center of said wide conduit and decreasing symmetrically to lower values of intensity at the two sides of said wide conduit; and

a delay activation circuit which activates said illumination profile in response to a signal derived from said visual inspection of said particles so as to cause an illuminated particle to be displaced from regions of maximum intensity to regions of lower intensity of said intensity profile and to be deflected into the intermittent spaces between said barriers.