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Digital karyotyping

  • US 20040096892A1
  • Filed: 11/13/2003
  • Published: 05/20/2004
  • Est. Priority Date: 11/15/2002
  • Status: Active Grant
First Claim
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1. A method of karyotyping a genome of a test eukaryotic cell, comprising:

  • generating a population of sequence tags from defined portions of the genome of the test eukaryotic cell, said portions being defined by one or two restriction endonuclease recognition sites;

    enumerating said sequence tags in the population to determine the number of individual sequence tags present in the population;

    comparing the number of a plurality of sequence tags in the population to the number of the plurality of sequence tags determined for a genome of a reference cell, wherein the plurality of sequence tags are within a window of sequence tags which are calculated to be contiguous in the genome of the species of the eukaryotic cell, wherein a difference in the number of the plurality of sequence tags within the window present in the population from the number determined for a reference eukaryotic cell indicates a karyotypic difference between the test eukaryotic cell and the reference eukaryotic cell.

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