Methods for preparing an electrosensor having a capture reagent
First Claim
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1. A device for detecting an analyte in a liquid sample, which device comprises:
- a) a solid support;
b) an electrosensor immobilized on said solid support, said electrosensor comprises a working electrode and another electrode used as auxiliary and/or reference electrode;
c) a capture reagent immobilized on said working electrode, said capture reagent is capable of binding to an analyte; and
d) conductive leads for connecting said electrodes to a readout device for electrochemical measurement.
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Abstract
The present invention relates to devices comprising electrosensors containing capture reagents, their preparation thereof, and their use for detecting, preferably, quantitative measurement, of analyte in a liquid sample. In particular, the invention relates to an enzyme electrosensor, e.g., electroimmunosensor, device for electrochemical detection and preferably, real-time measurement, which is suitable for use at point-of-care settings by unskilled personnel.
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Citations
74 Claims
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1. A device for detecting an analyte in a liquid sample, which device comprises:
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a) a solid support;
b) an electrosensor immobilized on said solid support, said electrosensor comprises a working electrode and another electrode used as auxiliary and/or reference electrode;
c) a capture reagent immobilized on said working electrode, said capture reagent is capable of binding to an analyte; and
d) conductive leads for connecting said electrodes to a readout device for electrochemical measurement. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 70)
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34. A device for detecting an analyte in a liquid sample, which device comprises:
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a) a base sensor strip having a working electrode, a reference electrode, and an auxiliary electrode coated on a plastic substrate, whereon a capture reagent is immobilized on the working electrode, said sensor strip having conductive leads for attaching the electrodes to a readout device for electrochemical measurement;
b) a cover casing having a liquid sample application aperture and a detection aperture;
c) an application zone for receiving a fluid containing an analyte from the application aperture, said application zone, in the dry unused form, containing a labeled detection reagent capable of specifically binding to said analyte, wherein the said labeled reagent is released into mobile form when in contact with the liquid sample;
d) a detection zone in fluid communication with the electrodes in the presence of a liquid sample received from the detection aperture;
e) a wicking member that carries the liquid sample from the application zone to the detection zone by capillary action, wherein said analyte is sandwiched between the detection reagent and the capture reagent immobilized on the electrode surface; and
f) an absorbent sink placed in partial contact with the wicking member at the end of the flow path to absorb any excess fluid from the detection zone. - View Dependent Claims (57)
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- 58. A method for preparing an electrochemical sensor for the detection of an analyte in a liquid sample, which method comprises immobilizing a capture reagent capable of binding to an analyte on the surface of a hydrophobic, non-metal electrode by contacting said electrode surface with a solution containing said capture reagent and an organic immobilizing agent that wets said electrode surface and facilitates immobilization of said capture regent on said electrode surface.
- 71. A device for detecting an analyte in a liquid sample, which device comprises a sample application area that is in fluid communication with an electrosensor via a wicking member, wherein the wicking member has an open mesh structure.
Specification