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Upon a cartridge for containing a specimen sample for optical analysis

  • US 20040101443A1
  • Filed: 11/25/2002
  • Published: 05/27/2004
  • Est. Priority Date: 11/25/2002
  • Status: Active Grant
First Claim
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1. An improved method for filling an analytic chamber with liquid by introducing said liquid into said chamber having a volume sufficient to fill said chamber to a level either up to or below a fill line, adding a buffer solution having a density different from the density of said liquid when below said fill line with the combined volume of said liquid and said buffer being sufficient to bring the surface of said combined liquids to said fill line, said test liquid and buffer solution providing a liquid interface within the test chamber with said buffer solution on one side and said liquid at the other end, then displacing said buffer solution during a seating step from the one side of said liquid interface into an overflow reservoir through a seal interface, and excluding air from said test chamber through a second seal to preclude voiding of said overflow reservoir, wherein the improvement comprises:

  • a. positioning said cartridge vertically;

    b. filling said test chamber with a fluid sample containing magnetically responsive particles and magnetically labeled target components within a magnetic field such that said test chamber is completely filled with said sample fluid; and

    c. storing said cartridge within said magnetic field for use in an apparatus to perform optical analysis.

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