Resonant cavity photodiode array for rapid DNA microarray readout

• US 20040101861A1
• Filed: 11/27/2002
• Published: 05/27/2004
• Est. Priority Date: 11/27/2002
• Status: Abandoned Application

• Current Assignee: Spire Corporation
• Original Assignee: Spire Corporation
• Inventors: Little, Roger G., Linden, Kurt J.
• Application Number:
  US10/307,219
• Publication Number:
  US 20040101861A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 2021/6469 Cavity, e.g. ellipsoid
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 2201/0407 with multiple optical units, e.g. one per sample
• CPC Class Codes: G01N 2201/0612 Laser diodes

Abstract

The present invention provides a microarray having a plurality of micro-locations for confining selected photophores, for example, biological molecules exhibiting fluorescence spectra. The microarray can further include an array of optoelectronic photodetectors each of which is optically coupled with at least one of the micro-locations to detect radiation, for example, fluorescence radiation, that is emitted from the photophores confined in that micro-location. Each photodetector includes a resonant cavity that is formed of a front reflector and/or a back reflector having distributed Bragg reflector structures and a photo-detecting element disposed in the resonant cavity. The microarray can utilize either external optical excitation sources, such as lasers, LEDs, or can contain its own excitation sources in an integrated structure containing both optical radiation emitters, such as, vertical cavity surface emitting lasers or resonant cavity LEDs, and resonant cavity photodetectors. The integrated emitters and detectors can be either coaxially or adjacently located. Further, the microarray can include either separate sample array and excitation/detector array plates, or a single sample/excitation/detector array plate in which the photophore-containing sample molecules can be deposited directly on the excitation/detector array.

Claims

1. A microarray, comprising:

a substrate having a plurality of micro-locations on a surface thereof, each of said micro-locations being capable of confining a photophore, and

an array of optoelectronic photodetectors, each having a resonant cavity comprising at least a reflector having distributed Bragg reflector (DBR) structure, being optically coupled to said substrate surface such that each of said photodetectors is optically coupled to at least one of said micro-locations to detect radiation originating therefrom.

2. The microarray of claim 1, wherein said reflector is a front reflector.

3. The microarray of claim 2, wherein said resonant cavity further comprises a back reflector having a DBR structure.

4. The microarray of claim 1, wherein each of said photodetectors exhibits a selected spectral response that at least partially overlaps with a frequency spectrum associated with radiation originating from the micro-location optically coupled to said photodetector.

5. The microarray of claim 1, wherein each of said photodetectors further comprises a photo-detecting element disposed in said resonant cavity.

6. The microarray of claim 3, wherein each of said photodetectors further comprises a photo-detecting element disposed between said front and back reflectors.

7. The microarray of claim 5, wherein said photo-detecting element can be any of a PIN photodiode, a phototransistor, an avalanche photodiode or a photodiode.

8. The microarray of claim 1, wherein a surface of each photodetector optically coupled to one of said microlocations substantially transmits radiation having one or more frequency components in a frequency range associated with radiation originating from said optically coupled micro-location.

9. The microarray of claim 3, wherein the front reflector of said photodetector substantially transmits radiation having one or more frequency components in a frequency range associated with radiation originating from the micro-location optically coupled to said photodetector.

10. The microarray of claim 9, wherein the back reflector of said photodetector substantially reflects radiation having one or more frequency components in the frequency range associated with radiation originating from the micro-location optically coupled to said photodetector.

11. The microarray of claim 1, wherein at least one of said photodetectors comprises a resonant photodiode detector.

12. The microarray of claim 1, wherein said array of photodetectors is formed in the substrate having the micro-locations.

13. The microarray of claim 1, wherein said array of photodetectors is formed in a substrate separate from the substrate having the micro-locations.

14. The microarray of claim 1, wherein each of said photodetectors exhibits a spectral response in a range of about 450 nm to about 1700 nm.

15. The microarray of claim 1, wherein the substrate having the micro-locations comprises any of glass or semiconductor.

16. The microarray of claim 1, wherein said photophore comprises a biological molecule having a fluorescence emission spectrum.

17. The microarray of claim 16, wherein the photodetector optically coupled to the micro-location confining said biological molecule exhibits a spectral response that at least partially overlaps said fluorescence spectrum.

18. The microarray of claim 16, wherein said biological molecule can be any of oligonucleotides, peptides, or peptide nucleic acids.

19. The microarray of claim 1, further comprising an excitation source optically coupled to said substrate for eliciting radiation from said photophore.

20. The microarray of claim 19, wherein said excitation source comprises a light source.

21. The microarray of claim 20, wherein said light source comprises any of a laser or an LED.

28. The method of claim 19, wherein said biological molecules can be any of oligonucleotides, peptides, or peptides nucleic acids.

22. A bioanalytical microarray, comprising

a substrate having a plurality of micro-locations on a surface thereof,

a plurality of biological molecules confined at said micro-locations, each biological molecule exhibiting a fluorescence spectrum, and

a plurality of optoelectronic photodetectors having resonant cavity structures comprising at least a reflector having distributed Bragg reflector structures and being optically coupled to said substrate such that each photodetector detects any of fluorescence and luminescence radiation originating from the molecules confined at one of said micro-locations.

23. The microarray of claim 22, wherein each of said photodetectors exhibits a spectral response function that at least partially overlaps any of the fluorescence and luminescence spectrum corresponding to radiation originating from molecules confined in one of said micro-locations and detected by said photodetector.

24. The microarray of claim 22, wherein said reflector is a front reflector.

25. The microarray of claim 24, wherein said resonant cavity further comprises a back reflector having a distributed Bragg reflector structure.

26. The microarray of claim 25, wherein each of said photodetectors comprises a photo-detecting element sandwiched between said front reflector and said back reflector.

27. The micro-array of claim 22, wherein each of said photodetectors comprises a resonant photodiode detector.

29. A method of processing radiation generated by a plurality of photophores, the method comprising the steps of:

disposing photophores on a plurality of micro-locations formed on a substrate surface, each micro-location being capable of confining at least one photophore type, and

optically coupling a plurality of optoelectronic photodetectors having resonant cavity structures comprising a front reflector and a back reflector having distributed Bragg reflector structures to said substrate surface to detect radiation generated by said photophores such that each photodetector detects radiation originating from one of said micro-locations.

30. The method of claim 29, further comprising selecting at least one of said photodetectors to be formed as a photo-detecting element sandwiched between the front reflector and the back reflector.

31. The method of claim 29, further comprising selecting said photophores to comprise biological molecules.

32. The method of claim 31, further comprising selecting said biological molecules from the group consisting of oligonucleotides, peptides, and peptide nucleic acids.

33. A microarray, comprising:

a substrate having a plurality of micro-locations on a surface thereof, each of said micro-locations being capable of confining a photophore, and

a plurality of optoelectronic photodetectors and emitters integrally formed in a single substrate and arranged as a plurality of emitter/detector pairs optically coupled to said micro-locations such that for each pair the emitter of the pair emits light for exciting photophores confined in at least one of said micro-locations and the detector of the pair detects radiation generated by the confined photophores in response to said excitation.

34. The microarray of claim 33, wherein at least one of said photodetectors includes a resonant cavity comprising at least a reflector having a distributed Bragg reflector (DBR) structure.

35. The microarray of claim 34, wherein said reflector is a front reflector.

36. The microarray of claim 35, wherein said resonant cavity further comprises a back reflector having a DBR structure.

37. The microarray of claim 36, wherein said at least one photodetector further comprises a photo-detecting element disposed between said front and back reflectors.

38. The microarray of claim 33, wherein each of said photo-emitters emit light having a spectrum that at least partially overlaps an absorption spectrum of the photophores confined in a micro-location optically coupled thereto.

39. The microarray of claim 38, wherein each of said photodetectors exhibits a selected spectral response that at least partially overlaps with a spectrum associated with radiation originating from the micro-location optically coupled thereto.

40. The microarray of claim 33, wherein the emitters associated with at least two different pairs exhibit different emission spectra.

41. The microarray of claim 37, wherein said photo-detecting element can be any of a PIN photodiode, a phototransistor, an avalanche photodiode or a photodiode.

42. The microarray of claim 33, wherein said photodetectors comprise distributed feedback laser diodes.

43. The microarray of claim 33, wherein each emitter/detector pair is formed in a substrate as an emitter coaxially surrounded by a detector.

44. The microarray of claim 33, wherein each emitter/detector pair is formed in a substrate as an emitter disposed proximate to a detector.

45. The microarray of claim 33, wherein said plurality of photodetectors and emitters form a two-dimensional array.

46. The microarray of claim 33, wherein said plurality of photodetectors and emitters form a one-dimensional array.