Surfactant/oxidizing agent solution and methods of use

• US 20040101881A1
• Filed: 12/15/2003
• Published: 05/27/2004
• Est. Priority Date: 02/01/2002
• Status: Abandoned Application

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Martinaitis, Christine, Durmowicz, Gerard, Poth, Tiffiny
• Application Number:
  US10/470,762
• Publication Number:
  US 20040101881A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

A solution is disclosed which is useful in removing contaminating nucleic acids from various surfaces, including areas such as laboratory bench tops, floors and walls, as well as surfaces of instruments and equipment in laboratories where nucleic acid amplification reactions are conducted. Further, methods and kits are provided that afford effective decontamination and removal of nucleic acids from such surfaces.

Claims

1. A solution for removing and helix-destabilizing nucleic acids on surfaces, consisting essentially of surfactant and oxidizing agent, said surfactant and oxidizing agent present in amounts sufficient to render nucleic acids unamplifiable and to permit greater than about 50% of amplification detectable nucleic acids to be removed from said surface.

2. The solution of claim 1, wherein said surfactant is a detergent.

3. The solution of claim 2, wherein said detergent is selected from the group consisting of anionic detergent, non-ionic detergent, ampholytic detergent and zwitterionic detergent.

4. The solution of claim 3, wherein said detergent consists essentially of sodium alkylaryl sulfonate, alcohol sulfate, phosphates and carbonates.

5. The solution of claim 1, wherein the concentration of surfactant is from about 0.5% to about 5%.

6. The solution of claim 5, wherein the concentration of surfactant is from about 0.6% to about 1%.

7. The solution of claim 1, wherein said oxidizing agent is a hypohalous acid or a peroxide.

8. The solution of claim 7, wherein said hypohalous acid is selected from the group consisting of sodium hypochlorite, lithium hypochlorite, calcium hypochlorite and dibasic magnesium hypochlorite.

9. The solution of claim 8, wherein said hypohalous acid is sodium hypochlorite.

10. The solution of claim 1, further comprising a buffer.

11. A kit comprising a first container consisting essentially of a surfactant and a second container consisting essentially of an oxidizing agent

12. The kit of claim 11, wherein said surfactant is a detergent.

13. The kit of claim 12, wherein said detergent is selected from the group consisting of anionic detergent, non-ionic detergent, ampholytic detergent and zwitterionic detergent.

14. The kit of claim 13, wherein said detergent consists essentially of sodium alkylaryl sulfonate, alcohol sulfate, phosphates and carbonates.

15. The kit of claim 11, wherein said oxidizing agent is a hypohalous acid or a peroxide.

16. The kit of claim 15, wherein said hypohalous acid is selected from the group consisting of sodium hypochlorite, lithium hypochlorite, calcium hypochlorite and dibasic magnesium hypochlorite.

17. The kit of claim 16, wherein said hypohalous acid is sodium hypochlorite.

18. A method of decontaminating surfaces previously exposed to amplifiable nucleic acids comprising contacting said surfaces with a solution, wherein said solution consists essentially of surfactant and oxidizing agent, and removing said solution from said surface.

19. The method of claim 18, wherein said surfactant is a detergent.

20. The method of claim 19, wherein said detergent is selected from the group consisting of anionic detergent, non-ionic detergent, ampholytic detergent and zwitterionic detergent.

21. The method of claim 20, wherein said detergent consists essentially of sodium alkylaryl sulfonate, alcohol sulfate, phosphates and carbonates.

22. The method of claim 18, wherein the concentration of surfactant is from about 0.5% to about 5%.

23. The method of claim 22, wherein the concentration of surfactant is from about 0.6% to about 1%.

24. The method of claim 18, wherein said oxidizing agent is a hypohalous acid or a peroxide.

25. The method of claim 24, wherein said hypohalous acid is selected from the group consisting of sodium hypochlorite, lithium hypochlorite, calcium hypochlorite and dibasic magnesium hypochlorite.

26. The method of claim 25, wherein said hypohalous acid is sodium hypochlorite.

27. The method of claim 18, wherein said surfaces are contacted with said solution for at least about 30 seconds.

28. The method of claim 18, wherein said surfaces are contacted with said solution for at least about two minutes.