Modification assisted profiling (MAP) methodology
First Claim
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1. A method of identifying a site of interaction between a first and second macromolecule, comprising:
- (a) immobilizing the first macromolecule onto at least two biosensor surfaces;
(b) treating each biosensor surface containing the immobilized first macromolecule with a different agent capable of altering the structure of the immobilized first macromolecule;
(c) exposing each treated biosensor surface to the second macromolecule;
(d) determining an interaction profile of the second macromolecule to the immobilized and treated first macromolecule; and
(e) identifying a site of interaction between the first and second macromolecules based on the interaction profile.
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Abstract
Methods of utilizing a biosensor platform for the purpose of studying macromolecule interactions is provided. Also provided are methods of sorting monoclonal antibodies directed against a particular antigen into functional groups wherein each group exhibits a characteristic binding profile to the antigen.
119 Citations
21 Claims
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1. A method of identifying a site of interaction between a first and second macromolecule, comprising:
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(a) immobilizing the first macromolecule onto at least two biosensor surfaces;
(b) treating each biosensor surface containing the immobilized first macromolecule with a different agent capable of altering the structure of the immobilized first macromolecule;
(c) exposing each treated biosensor surface to the second macromolecule;
(d) determining an interaction profile of the second macromolecule to the immobilized and treated first macromolecule; and
(e) identifying a site of interaction between the first and second macromolecules based on the interaction profile. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of sorting antigen-specific monoclonal antibodies (mAbs) into functional groups, comprising:
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(a) immobilizing the antigen onto at least two biosensor surfaces;
(b) treating each biosensor surface with a different agent, wherein each agent is capable of altering the structure of the immobilized antigen;
(c) exposing each treated biosensor surface to the antigen-specific mAbs;
(d) determining the binding profile of the monoclonal antibodies to each treated biosensor surface; and
(e) sorting the mAbs into functional groups based on a binding profile of the monoclonal antibodies to each treated biosensor surface, wherein mAbs that exhibit similar binding profiles to each treated sensor surface are sorted into the same functional group. - View Dependent Claims (18, 19, 20, 21)
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Specification