Enzyme electrode and process for manufacturing the same
First Claim
1. An enzyme electrode comprising:
- a portion of electrode formed on an insulating substrate;
an immobilized enzyme layer formed over the portion of electrode;
an adhesion layer comprising a silane-containing compound formed over the immobilized enzyme layer; and
a permeation-limiting layer comprising a fluorine-containing polymer having a structure where a pendant group containing at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer, which is formed on the adhesion layer.
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Abstract
The present invention provides an enzyme electrode exhibiting good measurement performance under wide ranges of the application conditions, being excellent in durability during long-term use and further being producible with a higher yield, as well as a process for manufacturing the enzyme electrode employing a wafer process particularly suitable to mass production. An enzyme electrode according to the present invention comprises an electrode 2 formed on an insulating substrate 1, an immobilized enzyme layer 4 formed over the electrode 2, and a permeation-limiting layer 6 placed on the uppermost surface and over the immobilized enzyme layer 4, wherein on the immobilized enzyme layer 4 is optionally formed an adhesion layer 8 comprising a silane-containing compound, on whose upper surface is formed the permeation-limiting layer 6; or the permeation-limiting layer 6 may be a film mainly comprising a fluorine-containing polymer in which a number of grooves are built on its surface, or alternatively the film has an irregular surface having a surface roughness of 0.0001 or more and 1 or less fold to its average thickness being selected within a range of 0.01 to 1 μm.
26 Citations
34 Claims
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1. An enzyme electrode comprising:
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a portion of electrode formed on an insulating substrate;
an immobilized enzyme layer formed over the portion of electrode;
an adhesion layer comprising a silane-containing compound formed over the immobilized enzyme layer; and
a permeation-limiting layer comprising a fluorine-containing polymer having a structure where a pendant group containing at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer, which is formed on the adhesion layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 28)
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8. An enzyme electrode comprising:
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a portion of electrode formed on an insulating substrate;
an electrode protective layer covering the portion of electrode;
a binding layer comprising a silane-containing compound, which is formed on the electrode protective layer;
an ion-exchange resin film layer formed on the binding layer;
an immobilized enzyme layer formed on the ion-exchange resin film layer;
an adhesion layer comprising a silane-containing compound, which is formed on the immobilized enzyme layer; and
a permeation-limiting layer comprising a fluorine-containing polymer having a structure where a pendant group containing at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer, which is formed on the adhesion layer. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15)
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16. An enzyme electrode comprising:
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a portion of electrode formed on an insulating substrate;
an immobilized enzyme layer formed on the portion of electrode; and
a permeation-limiting layer formed over the immobilized enzyme layer and placed on the uppermost surface;
wherein said permeation-limiting layer consists of a film essentially comprising a fluorine-containing polymer, and many grooves are built in on the surface of said permeation-limiting layer consisting of the film essentially comprising a fluorine-containing polymer. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. A process for manufacturing an enzyme electrode comprising the steps of:
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forming an electrode film on the main surface of an insulating substrate and then patterning the electrode film to form a plurality of portions of electrodes;
forming an electrode protective layer covering the electrode surface;
forming a binding layer comprising a silane-containing compound on the main surface of the insulating substrate;
forming an ion-exchange resin film layer on the main surface of the insulating substrate;
applying an liquid containing an enzyme to the main surface of the insulating substrate and then drying the insulating substrate to form an immobilized enzyme layer;
applying a liquid containing a fluorine-containing polymer having a structure where a pendant group comprising at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer to the main surface of the insulating substrate by spin coating and then drying the insulating substrate to form the permeation-limiting layer; and
dicing the insulating substrate to give a plurality of enzyme electrodes. - View Dependent Claims (25, 26, 27, 29, 30, 31, 33, 34)
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32. An process for manufacturing an enzyme electrode as claimed in claim 32 wherein said fluorine-containing polymer is a copolymer of said fluoroalcohol ester of the polycarboxylic acid (A) and the alkylalcohol ester of the polycarboxylic acid (B).
Specification