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Method for making hematopoietic cells

  • US 20040110286A1
  • Filed: 12/06/2002
  • Published: 06/10/2004
  • Est. Priority Date: 12/06/2002
  • Status: Abandoned Application
First Claim
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1. A method for differentiating human pluripotent stem (hPS) cells into a cell population with hematopoietic potential, comprising:

  • a) harvesting undifferentiated hPS cells from a feeder-free culture;

    b) differentiating the harvested hPS cells in a culture environment essentially free of any cells having a different genotype, but containing at least two hematopoietic growth factors selected from stem cell factor (SCF), FLT-3 ligand, IL-3, IL-6, and granulocyte colony stimulating factor (G-CSF);

    c) simultaneously or subsequently culturing the differentiating cells with a bone morphogenic protein; and

    d) harvesting from the culture environment a cell population that is at least 1% CD45 positive, or that forms colonies in an assay for hematopoietic colony forming units (CFU) at a plating efficiency of at least −

    1 in 2000.

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