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Method for base sequencing and biologically active nucleic acids

  • US 20040115665A1
  • Filed: 08/14/2003
  • Published: 06/17/2004
  • Est. Priority Date: 11/28/2000
  • Status: Active Grant
First Claim
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1. A method for preparing nucleic acid aptamers comprising at least one base capable of base pairing and different from the standard Watson-Crick (W-C) base comprising:

  • a) providing a specific ligand;

    b) synthesizing a pool of nucleic acid aptamers comprising at least one base capable of base pairing and different from the standard W-C bases;

    c) mixing the pool of aptamers with the specific ligand;

    d) selecting and amplifying a specific aptamer that binds to the specific ligand.

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