Suppression of non-specific nucleic acid amplication
First Claim
Patent Images
1. ) A method of suppressing background signal in an isothermal nucleic acid amplification reaction wherein at least one of the primers used comprises at least one of a nucleotide analogue a hairpin loop at the 5′
- end of the primer a ribonucleotide a fluor or quencher
2 Assignments
0 Petitions
Accused Products
Abstract
The invention discloses methods of reducing background signal in nucleic acid amplification reactions by the use of primers in the case of isothermal amplification which include at least one modification selected from a nucleotide analogue, a hairpin loop at the 5′ end of the primer, a ribonucleotide or a fluor or quencher. For more general nucleic acid amplification reactions the primer includes at least two of the modifications.
-
Citations
6 Claims
-
1. ) A method of suppressing background signal in an isothermal nucleic acid amplification reaction wherein at least one of the primers used comprises at least one of
a nucleotide analogue a hairpin loop at the 5′ - end of the primer
a ribonucleotide a fluor or quencher - View Dependent Claims (3, 4, 5, 6)
- end of the primer
-
2. ) A method of suppressing background signal in a nucleic acid amplification reaction wherein the primer comprises at least two of
a nucleotide analogue a hairpin loop at the 5′ - end of the primer
a ribonucleotide a fluor or quencher
- end of the primer
Specification