Suppression of non-specific nucleic acid amplication
First Claim
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1. ) A method of suppressing background signal in an isothermal nucleic acid amplification reaction wherein at least one of the primers used comprises at least one of a nucleotide analogue a hairpin loop at the 5′
- end of the primer a ribonucleotide a fluor or quencher
2 Assignments
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Accused Products
Abstract
The invention discloses methods of reducing background signal in nucleic acid amplification reactions by the use of primers in the case of isothermal amplification which include at least one modification selected from a nucleotide analogue, a hairpin loop at the 5′ end of the primer, a ribonucleotide or a fluor or quencher. For more general nucleic acid amplification reactions the primer includes at least two of the modifications.
17 Citations
6 Claims
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1. ) A method of suppressing background signal in an isothermal nucleic acid amplification reaction wherein at least one of the primers used comprises at least one of
a nucleotide analogue a hairpin loop at the 5′ - end of the primer
a ribonucleotide a fluor or quencher - View Dependent Claims (3, 4, 5, 6)
- end of the primer
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2. ) A method of suppressing background signal in a nucleic acid amplification reaction wherein the primer comprises at least two of
a nucleotide analogue a hairpin loop at the 5′ - end of the primer
a ribonucleotide a fluor or quencher
- end of the primer
Specification
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Current AssigneeGE Healthcare UK Limited (Danaher Corporation)
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Original AssigneeAmersham Biosciences (General Electric Company)
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InventorsSmith, Clifford, Schwarz, Terek, Pickering, Judith, Knott, Tim
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Application NumberUS10/466,580Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/6CPC Class CodesC12Q 1/6848 characterised by the means ...C12Q 2525/113 incorporating modified back...C12Q 2525/121 incorporating both deoxyrib...C12Q 2525/301 Hairpin oligonucleotidesC12Q 2531/125 Rolling circleC12Q 2565/1015 labels being on the same ol...
