Method and apparatus for analyzing a base sequence
First Claim
1. A method for analyzing a base sequence, comprising the steps of forming a thin film for immobilizing a base sequence test sample, on the front surface of a first board;
- stretching and immobilizing a base sequence test sample on the thin film;
cutting the base sequence test sample in this state into fragments by means of an enzyme;
heating and vaporizing the thin film in a desired region by a heating means, to shoot the fragment of the base sequence test sample in the desired region from the front surface of the first board, in order that the fragment can be arrested on the front surface of a second board disposed in opposite to the front surface of the first board; and
analyzing the base sequence in this state.
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Accused Products
Abstract
Proposed is a method comprising the steps of stretching and arranging DNA while immobilizing it on a board, cutting it into fragments sequentially from one end, analyzing the respective fragments, connecting the analyzed results for analyzing the entire base sequence of the original DNA. For practically applying this method, required is a method of efficiently recovering DNA fragments without disturbing the original base sequence.
The present invention proposes a method for analyzing a base sequence, comprising the steps of forming a thin film 3 for immobilizing a base sequence test sample on the front surface of a first board 1; stretching and immobilizing a base sequence test sample 4 on the thin film; cutting the base sequence test sample in this state into fragments by means of an enzyme; heating and vaporizing the thin film in a desired region by a heating means, to shoot the fragment 7 of the base sequence test sample in the desired region from the front surface of the first board 1, in order that the fragment 7 can be arrested on the front surface of a second board 8 disposed in opposite to the front surface of the first board 1; and analyzing the base sequence in this state.
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Citations
25 Claims
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1. A method for analyzing a base sequence, comprising the steps of forming a thin film for immobilizing a base sequence test sample, on the front surface of a first board;
- stretching and immobilizing a base sequence test sample on the thin film;
cutting the base sequence test sample in this state into fragments by means of an enzyme;
heating and vaporizing the thin film in a desired region by a heating means, to shoot the fragment of the base sequence test sample in the desired region from the front surface of the first board, in order that the fragment can be arrested on the front surface of a second board disposed in opposite to the front surface of the first board; and
analyzing the base sequence in this state. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9)
- stretching and immobilizing a base sequence test sample on the thin film;
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2. A method for analyzing a base sequence, comprising the steps of forming a thin film for immobilizing a base sequence test sample, on an ablation layer containing a material capable of being vaporized by heating, formed on the front surface of a first board;
- stretching and immobilizing a base sequence test sample on the ablation layer;
cutting the base sequence test sample in this state into fragments by means of an enzyme;
heating and vaporizing the ablation layer in a desired region by a heating means, to shoot the fragment of the base sequence test sample in the desired region from the front surface of the first board, in order that the fragment can be arrested on the front surface of a second board disposed in opposite to the front surface of the first board; and
analyzing the base sequence in this state. - View Dependent Claims (10, 11, 12, 13)
- stretching and immobilizing a base sequence test sample on the ablation layer;
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14. An apparatus for analyzing a base sequence, comprising a first board having a thin film formed on its front surface for allowing a base sequence test sample to be stretched and immobilized on the thin film;
- a heating means for heating and vaporizing the thin film in a desired region; and
a second board disposed in opposite to the front surface of the first board. - View Dependent Claims (16, 17, 18, 19, 20, 21)
- a heating means for heating and vaporizing the thin film in a desired region; and
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15. An apparatus for analyzing a base sequence, comprising a first board having a thin film for allowing a base sequence test sample to be stretched and immobilized, formed on an ablation layer containing a material capable of being vaporized by heating, formed on the front surface of the first board;
- a heating means for heating and vaporizing the ablation layer in a desired region; and
a second board disposed in opposite to the front surface of the first board. - View Dependent Claims (22, 23, 24, 25)
- a heating means for heating and vaporizing the ablation layer in a desired region; and
Specification