Electroprocessed collagen

• US 20040116032A1
• Filed: 07/29/2003
• Published: 06/17/2004
• Est. Priority Date: 02/25/1999
• Status: Abandoned Application

• Current Assignee: David G. Simpson, Gary L. Bowlin, Gary Wnek
• Original Assignee: David G. Simpson, Gary L. Bowlin, Gary Wnek
• Inventors: Simpson, David G., Bowlin, Gary L., Wnek, Gary
• Application Number:
  US10/630,624
• Publication Number:
  US 20040116032A1
• Time in Patent Office: Days
• US Class Current: 442/414
• CPC Class Codes: A61F 2/08 Muscles; Tendons; Ligaments sutures A61B17/04
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61L 15/32 Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin A61L15/225 takes precedence
• CPC Class Codes: A61L 2430/30 for muscle reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: B29C 41/006 using an electrostatic field for applying the material
• CPC Class Codes: B29C 67/0007 Manufacturing coloured articles not otherwise provided for, e.g. by colour change
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0012 Cell encapsulation
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: D01D 5/0038 : the fibre formed by solvent evaporation, i.e. dry electro-spinning
• CPC Class Codes: D01F 4/00 : Monocomponent artificial filaments or the like of proteins; Manufacture thereof
• CPC Class Codes: Y10T 442/696 : Including strand or fiber material which is stated to have specific attributes [e.g., heat or fire resistance, chemical or solvent resistance, high absorption for aqueous compositions, water solubility, heat shrinkability, etc.]

Abstract

A matrix of collagen is obtained through the method of electroprocessing. As a common natural polymer, collagen may be electroprocessed to form a matrix for multiple different applications.. The flexibility and variability of the processing allows the collagen matrix to be predesigned to meet many applications. These applications are included, but not limited to, biomedical applications, manufactured leather applications, food casing products, and footwear and clothing products.

Claims

1. The product of the process of electroprocessing collagen.

2. The product described in claim 1, wherein the process of electroprocessing comprises electrospinning collagen fibers.

3. The product described in claim 1, wherein the process of electroprocessing comprises electrospraying collagen droplets.

4. The product described in claim 1, wherein the collagen comprises synthetically manufactured collagen.

5. A method for making a matrix of collagen comprising:

providing a substrate,

providing a reservoir of solution comprising collagen wherein the reservoir has an orifice that allows the solution to leave the reservoir,

electrically charging either the substrate or the solution, and grounding the other of the substrate or the solution that is not electrically charged, and streaming the collagen onto the substrate to form a matrix.

6. The method described in claim 5, wherein the step of streaming the collagen onto the substrate forms a matrix of collagen fibers.

7. The method described in claim 5, wherein the step of streaming the collagen onto the substrate forms a matrix of collagen droplets.

8. The method described in claim 5 wherein the substrate defines a preselected shape.

9. The method described in claim 5, further comprising treating the collagen matrix with a cross-linking agent.

10. The method described in claim 5, wherein the collagen comprises synthetically manufactured collagen.

11. The method described in claim 5, wherein the collagen comprises a subset of a collagen molecule.

12. A method for making a matrix of collagen comprising:

providing a substrate,

providing a target,

providing a reservoir of solution comprising collagen wherein the reservoir has an orifice that allows the solution to leave the reservoir,

electrically charging either the target or the solution, and grounding the other of the target or solution that is not electrically charged,

disposing the substrate between the orifice and the target, and streaming the collagen onto the substrate to form a matrix.

13. The method described in claim 12, wherein the step of streaming the collagen onto the substrate forms a matrix of collagen fibers.

14. The method described in claim 12, wherein the step of streaming the collagen onto the substrate forms a matrix of collagen droplets.

15. The method described in claim 12, wherein the substrate defines a preselected shape.

16. The method described in claim 12, further comprising treating the collagen matrix with a cross-linking agent.

17. The method described in claim 12, wherein the collagen comprises synthetically manufactured collagen.

18. The method described in claim 12, wherein the collagen comprises a subset of a collagen molecule.

19. A food casing comprising a matrix of electroprocessed collagen.

20. The food casing described in claim 19, wherein the collagen comprises electrospun collagen fibers.

21. The food casing described in claim 19, wherein the collagen comprises electrosprayed collagen droplets.

22. The food casing described in claim 19, wherein the collagen is cross-linked.

23. A method of making a food casing comprising electroprocessing a matrix of collagen.

24. Manufactured leather comprising a matrix of electroprocessed collagen.

25. The manufactured leather described in claim 24, wherein the collagen comprises electrospun collagen fibers.

26. The manufactured leather described in claim 24, wherein the collagen comprises electrosprayed collagen droplets.

27. The manufactured leather described in claim 24, wherein the collagen is cross-linked.

28. A method of manufacturing leather comprising electroprocessing a matrix of collagen.

29. The method described in claim 28, further comprising the step of treating the collagen matrix with a cross-linking agent.