Needleless vaccination using chimeric yellow fever vaccine-vectored vaccines against heterologous flaviviruses

• US 20040120964A1
• Filed: 10/02/2003
• Published: 06/24/2004
• Est. Priority Date: 10/29/2001
• Status: Abandoned Application

• Current Assignee: Andrea Waterston, Cheryl Dean, Farshad Guirakhoo, Jason B. Alarcon, John A. Mikszta, Monath P. Thomas
• Original Assignee: Andrea Waterston, Cheryl Dean, Farshad Guirakhoo, Jason B. Alarcon, John A. Mikszta, Monath P. Thomas
• Inventors: Guirakhoo, Farshad, Thomas, Monath P., Alarcon, Jason B., Mikszta, John A., Waterston, Andrea, Dean, Cheryl
• Application Number:
  US10/679,036
• Publication Number:
  US 20040120964A1
• Time in Patent Office: Days
• US Class Current: 424/184.1
• CPC Class Codes: A61B 17/54 Chiropodists' instruments , e.g. pedicure chiropractic devices A61H1/008
• CPC Class Codes: A61B 2017/320004 abrasive
• CPC Class Codes: A61K 2039/5256 expressing foreign proteins
• CPC Class Codes: A61K 2039/54 characterised by the route of administration
• CPC Class Codes: A61K 2039/545 characterised by the dose, timing or administration schedule
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61M 2037/0007 having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents
• CPC Class Codes: A61M 2037/0023 Drug applicators using microneedles
• CPC Class Codes: A61M 2037/0046 Solid microneedles
• CPC Class Codes: A61M 2037/0061 Methods for using microneedles
• CPC Class Codes: A61M 31/002 Devices for releasing a drug at a continuous and controlled rate for a prolonged period of time artificial gland structures or devices A61F2/022; intra-uterine contraceptive devices A61F6/14; tampons for introducing into the vagina A61F13/20, A61L15/00; suppositories or bougies for intra-vaginal or intra-uterine application A61K9/02; physical forms of medicinal preparations for sustained or differential drug release A61K9/20, A61K9/50
• CPC Class Codes: A61M 37/0015 by using microneedles
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2770/24122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/24134 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/24143 viral genome or elements thereof as genetic vector
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates to a method of vaccinating a subject comprising delivering a chimeric yellow fever 17D strain vector expressing an envelope protein gene product of a heterologous flavivirus to the epidermal compartment or the intradermal compartment of the subject'"'"'s skin. The invention encompasses vaccine compositions comprising the chimeric yellow fever viruses expressing an envelope protein gene product of a heterologous flavivirus. The compositions of the invention result in an enhanced therapeutic efficacy, e.g., enhanced protective immune response as they enhance the presentation and availability of the chimeric vaccine to the targeted compartment of the subject'"'"'s skin.

Claims

1. A method for inducing an immune response to a flavivirus viral antigen in a subject, comprising delivering a chimeric yellow fever virus expressing an envelope gene product of a flavivirus virus to a subject'"'"'s skin using a device that abrades an epidermal layer of the skin'"'"'s surface while delivering the chimeric virus.

2. The method of claim 1, wherein the flavivirus is Japanese encephalitis.

3. The method of claim 1, wherein the flavivirus is Dengue.

4. The method of claim 1, wherein the chimeric virus is delivered to an epidermal compartment of the subject'"'"'s skin.

5. The method of claim 1, wherein the chimeric virus is delivered to an immune cell in the subject'"'"'s skin.

6. The method of claim 5, wherein the immune cell is a dendritic cell or a Langerhan cell.

7. The method of claim 1, wherein the device is needleless.

8. The method of claim 1, wherein the device is a microabrader device.

9. The method of claim 1, wherein the device comprises at least one microneedle.

10. The method of claim 1 wherein the subject is a human.

11. A kit for use in inducing an immune response to a flavivirus viral antigen in a subject, said kit comprising:

(a) a chimeric yellow fever virus expressing an envelope gene product of a flavivirus and (b) a device that abrades an epidermal layer of the skin'"'"'s surface while delivering the chimeric virus.

12. The kit of claim 11, wherein the device is needleless.

13. The kit of claim 11, wherein the device is a microabrader device.

14. The kit of claim 11, wherein the device comprises at least one microneedle.

15. The kit of claim 11, wherein the flavivirus is Japanese Encephalitis or Dengue.