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Cryopreservation of sperm

  • US 20040121303A1
  • Filed: 12/10/2003
  • Published: 06/24/2004
  • Est. Priority Date: 08/10/2000
  • Status: Abandoned Application
First Claim
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1. A method of providing sperm comprising:

  • a) cooling a sample which includes sperm to a first temperature sufficient to protect sperm from glycerol toxicity, at a rate sufficiently slow that the metabolic rate of sperm is decreased, to provide a cooled sperm sample;

    b) adding a solution comprising glycerol to the cooled sperm sample; and

    c) freezing said cooled sperm sample to second temperature for a sufficient period of time to equilibrate glycerol and sperm to thereby provide a frozen sperm sample, such that the sperm is preserved.

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