Generation of efficacy, toxicity and disease signatures and methods of use thereof
First Claim
1. A method of generating a small molecule efficacy or toxicity profile for an agent, toxicant, or drug, wherein the profile is generated from a subject treated with said agent, toxicant, or drug, said method comprising the steps of:
- a. isolating from said treated subject, a biological sample selected from the group consisting of organ, tissue, cell, cellular compartment, organelle, cerebrospinal fluid, synovial fluid, blood and urine;
b. extracting small molecules from said biological sample; and
c. analyzing said small molecules from said sample by mass spectroscopy, wherein analyzing said small molecules results in the generation of a small molecule efficacy or toxicity profile.
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Abstract
The invention provides methods of generating small molecule efficacy profiles and signature, small molecule toxicity profiles and signatures and small molecule disease profiles and signatures. The invention also provides methods of determining the efficacy and/or toxicity of unknown agents and drugs in a subject and methods of diagnosing an unknown disease or disorder in a subject. The invention further provides methods of monitoring the progression or remission of a disease or disorder in a subject undergoing treatment and methods of measuring the effectiveness of treatment.
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Citations
45 Claims
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1. A method of generating a small molecule efficacy or toxicity profile for an agent, toxicant, or drug, wherein the profile is generated from a subject treated with said agent, toxicant, or drug, said method comprising the steps of:
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a. isolating from said treated subject, a biological sample selected from the group consisting of organ, tissue, cell, cellular compartment, organelle, cerebrospinal fluid, synovial fluid, blood and urine;
b. extracting small molecules from said biological sample; and
c. analyzing said small molecules from said sample by mass spectroscopy, wherein analyzing said small molecules results in the generation of a small molecule efficacy or toxicity profile. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 33, 34, 35, 36, 37, 38, 39, 40)
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21. A method of generating a small molecule disease profile from a subject suffering from a known or unknown disease or disorder, said method comprising the steps of:
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a. isolating from said diseased subject, a biological sample selected from the group consisting of organ, tissue, cell, cellular compartment, organelle, cerebrospinal fluid, synovial fluid, blood and urine;
b. extracting small molecules from said biological sample; and
c. analyzing said small molecules from said sample by mass spectroscopy, wherein analyzing said small molecules results in the generation of a small molecule disease profile. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 41, 42, 43, 44)
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45. A method of monitoring the progression or remission of a disease or disorder in a subject undergoing treatment for said disease or disorder, said method comprising the steps of:
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a. obtaining a small molecule disease profile from said subject at the onset of treatment for said disease or disorder;
b. obtaining small molecule disease profiles from said subject at multiple times during the course of treatment for said disease or disorder; and
c. comparing the small molecule disease profiles from step b) with the small molecule disease profile obtained at the onset of treatment, thereby measuring the effectiveness of said treatment and monitoring the progression or remission of said disease or disorder in said subject.
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Specification