Luminescent polymers and methods of use thereof
First Claim
1. An article, comprising:
- a composition comprising a luminescent polymer and a recognition entity, wherein the composition is a sol, a blend, or a film.
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Abstract
The present invention involves a series of articles, compositions, methods, and kits. Some aspects of the invention include articles such as particles, sols, blends, dispersions, films, or microarrays that comprise luminescent polymers, as well as methods for making and using such articles. In some cases, the luminescent polymer may be characterized in part by having a delocalized π-orbital structure, which can allow the polymer to have a high degree of luminosity. The polymers of this invention may also have, in some embodiments, bulky substituents to prevent intermolecular π-π interactions that can decrease luminosity. Some articles may include more than one luminescent polymer, for example, a first polymer that absorbs energy and directs the energy to a second polymer that releases the energy. The articles of the invention may be used, in certain embodiments, to detect the presence of other compounds such as single molecules, proteins, or specific nucleic acid sequences, as well as cells, bacteria, and viruses. In one set of embodiments, the luminescent polymers are associated with an entity that can interact with, for example, a nucleic acid or a protein. The association may be direct, or through an energy transfer pathway. The entity can be, for example, a nucleic acid, a charged surface, an intercalating agent, or an entity that releases a quenching agent that interacts with the luminescent polymer.
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Citations
91 Claims
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1. An article, comprising:
a composition comprising a luminescent polymer and a recognition entity, wherein the composition is a sol, a blend, or a film. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. An article, comprising:
a sol comprising a luminescent polymer. - View Dependent Claims (35, 36, 37)
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38. An article, comprising:
a blend comprising a luminescent polymer. - View Dependent Claims (39, 40)
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41. An article, comprising:
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a mixture comprising a first polymer having a first excitation wavelength and a first emission wavelength;
a second polymer having a second excitation wavelength and a second emission wavelength different from the first emission wavelength; and
an energy migration pathway between the first polymer and the second polymer;
wherein the mixture is able to emit light at substantially the second emission wavelength when incident light at the first excitation wavelength is applied to the composition.
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42. A method, comprising:
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providing a homogeneous composition comprising a first polymer and a second polymer different from the first polymer;
exposing the composition to energy that is substantially absorbed by the first polymer but is not substantially absorbed by the second polymer; and
detecting light emitted from the composition, wherein the light is emitted substantially by the second polymer.
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43. An article, comprising:
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a luminescent polymer;
a recognition entity selected from the group consisting of a nucleic acid recognition entity, a protein recognition entity, and an aptamer; and
an energy migration pathway between the luminescent polymer and the recognition entity. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60)
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61. An article, comprising:
a substrate having a surface charge, the substrate comprising a luminescent polymer. - View Dependent Claims (62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77)
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78. A method, comprising:
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providing a article comprising a luminescent polymer and a nucleic acid recognition entity, the article having a luminosity;
allowing a nucleic acid molecule to bind to the nucleic acid recognition entity; and
detecting a change in luminosity of the article. - View Dependent Claims (79, 80, 81, 82)
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83. An article, comprising:
a microarray comprising a luminescent polymeric composition, wherein the composition is a particle, a sol, a blend, or a film.
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84. An article, comprising:
a composition comprising a luminescent polymer and an aptamer. - View Dependent Claims (85, 86, 87, 88, 89, 90, 91)
Specification