Special film-coated substrate for bio-microarray fabrication and use thereof

• US 20040121339A1
• Filed: 12/19/2002
• Published: 06/24/2004
• Est. Priority Date: 12/19/2002
• Status: Abandoned Application

• Current Assignee: UT-Battelle LLC
• Original Assignee: UT-Battelle LLC
• Inventors: Zhou, Jizhong, Zhou, Xichun
• Application Number:
  US10/326,031
• Publication Number:
  US 20040121339A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C03C 17/009 Mixtures of organic and inorganic materials, e.g. ormosils and ormocers
• CPC Class Codes: C03C 17/3405 with at least two coatings of organic materials C03C17/36, C03C17/42 take precedence
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof

Abstract

Platforms for easy and cost-effective fabrication of bio-microarrays are disclosed. In one embodiment, the platform contains a substrate having a surface coated with a film of alternating polycationic and polyanionic polymers. In another embodiment, the platform contains a substrate having a surface coated with a polyelectrolyte-silica composite film. Also disclosed are bio-microarrays fabricated using the above platforms and methods of making the platforms and the microarrays.

Claims

1. A platform for fabricating a bio-microarray, the platform comprising a solid substrate and a film coating a surface of the solid substrate wherein the film comprises alternating polycationic and polyanionic polymer layers.

2. The platform of claim 1, wherein the solid substrate is made of glass, silica or plastic.

3. The platform of claim 1, wherein the polycationic polymer is selected from polyallylamine hydrochloride (PAAH), polyethyleneimine (PEI), polydimethyldiallylammonium chloride (PDDA), polyacrylamide (PAAM), poly(4-vinylpyridine) hydrochloride (PVP), or an analog of any of the foregoing polymers.

4. The platform of claim 1, wherein the polyanionic polymer is selected from polystyrenesulfonate (PSS), polyvinylsulfonate (PVS), dextrinsulfate (DTS), or an analog of any of the foregoing polymers.

5. The platform of claim 1, wherein the film comprises a pair of a polycationic and polyanionic polymers selected from PAAH/PSS, PAAH/DTS, PEI/PSS, PEI/PVS, PDDA/PSS, PDDA/PVS or PDDA/DTS.

6. The platform of claim 1, wherein the film further comprises metal or NH₄ ions.

7. The platform of claim 6, wherein the metal ions are selected from Mn²⁺, Cu²⁺, Fe²⁺, Na⁺, NH₄⁺, K⁺, Ni²⁺ or Mg²⁺.

8. A bio-microarray comprising the platform of claim 1 and a biological matter attached to the film of the platform.

9. The bio-microarray of claim 8, wherein the biological matter is a biomolecule selected from a polynucleotide or a polypeptide.

10. The bio-microarray of claim 8, wherein the biological matter is selected from a cellular organelle, a cell or a tissue sample.

11. A method for making the platform of claim 1 comprising the steps of:

providing a solid substrate suitable for fabricating bio-microarrays;

coating a surface of the solid substrate with a layer of a first polyionic polymer; and

coating the layer of the first polyionic polymer on the substrate with a layer of a second polyionic polymer wherein the ions of the second polyionic polymer is of the opposite charge to the ions of the first polyionic polymer.

12. The method of claim 11, wherein the coating steps involve exposing the substrate to a solution containing a polyionic polymer.

13. The method of claim 12, wherein the solution is an aqueous solution.

14. The method of claim 12, further comprising the step of adding a salt into the polyionic polymer solution before exposing the solid substrate to the solution.

15. The method of claim 14 wherein the salt is an inorganic salt.

16. A method for fabricating a bio-microarray comprising the steps of:

providing a platform for fabricating a bio-microarray according to claim 1;

and

attaching a biological matter to the film of the platform.

17. A platform for fabricating a bio-microarray, the platform comprising a solid substrate and a polyelectrolyte-silica composite film coating a surface of the solid substrate.

18. The platform of claim 17, wherein the polyelectrolyte-silica composite film is formed using a silica sol-gel material that comprises a molecule selected from aminoalkylsiloxanes, aminocarboxyalkylsiloxanes, carboxyalkylsiloxanes or alkoxaysilanes.

19. The platform of claim 17, wherein the solid substrate is made of glass, silica or plastic.

20. The platform of claim 17, wherein the polyelectrolyte is selected from polyallylamine hydrochloride (PAAH), polyethyleneimine (PEI), polydimethyldiallylammonium chloride (PDDA), polyacrylamide (PAAM), poly(4-vinylpyridine) hydrochloride (PVP), polystyrenesulfonate (PSS), polyvinylsulfonate (PVS), dextrinsulfate (DTS), or an analog of any of the foregoing polymers.

21. A bio-microarray comprising the platform of claim 17 and a biological matter attached to the film of the platform.

22. The bio-microarray of claim 21, wherein the biological matter is a biomolecule selected from a polynucleotide or a polypeptide.

23. The bio-microarray of claim 21, wherein the biological matter is selected from a cellular organelle, a cell or a tissue sample.

24. A method for making the platform of claim 17 comprising the steps of:

providing a solid substrate suitable for fabricating bio-microarrays; and

coating a surface of the solid substrate with a polyelectrolyte-silica composite film.

25. The method of claim 24, wherein the coating step involves exposing the substrate to a sol-gel silica solution doped with a polyelectrolyte.

26. The method of claim 24, wherein the polyelectrolyte-silica composite film is coated onto the surface of the solid substrate by the spin-coating method.

27. A method for fabricating a bio-microarray comprising the steps of:

providing a platform for fabricating a bio-microarray according to claim 17;

and

attaching a biological matter to the film of the platform.