Selective covalent-binding compounds having therapeutic diagnostic and analytical applications
First Claim
1. A compound for specifically binding a target molecular structure, comprising a chemically modified reactive compound that is selective for the target, having an enhanced apparent affinity constant at least double that of the chemically unmodified parent compound.
1 Assignment
0 Petitions
Accused Products
Abstract
Novel compounds are provided having enhanced affinity for a desired, preselected, target substance (a small molecule; a macromolecule such as a protein, a carbohydrate, a nucleic acid, a cell, a viral particle, etc.) by modification with chemical groups that allow these substances to form strong bonds, such as irreversible covalent bonds, with the desired target substance. These qualities of tight, specific binding are reminiscent of antibody-like affinity; hence the new substances are termed COBALT, an acronym for Covalent-Binding Antibody-Like Trap. The present invention includes a process wherein a target species is chosen and then, by synthetic chemical procedures and modifications, novel substances (COBALTs) are obtained that exhibit selective and covalent binding to the preselected target species. The applications of the COBALTs include diagnostic, analytical, therapeutic and industrial applications.
-
Citations
64 Claims
- 1. A compound for specifically binding a target molecular structure, comprising a chemically modified reactive compound that is selective for the target, having an enhanced apparent affinity constant at least double that of the chemically unmodified parent compound.
-
30. A method for creating a compound for specifically binding a target molecular structure, the compound comprising a selective and chemically reactive compound with an enhanced apparent affinity constant, the method comprising:
-
providing a combinatorial library of compounds containing chemically reactive groups;
screening said combinatorial library for a compound having a selective chemical reaction with the target molecular structure; and
creating the compound for specifically binding the target molecular structure from at least said compound of said combinatorial library. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 59, 60, 61, 62, 64)
-
-
38. A method for creating a compound for specifically binding a target molecular structure, the method comprising:
-
preparing an MIP for binding to the target molecular structure; and
converting at least a portion of said MIP to an active functional group for forming a covalent bond with the target molecular structure upon binding of said MIP to the target molecular structure, thereby forming the compound for binding the target molecular structure. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46)
-
-
47. A method for creating a compound for specifically binding a chemically activated target molecular structure, the method comprising:
-
preparing an MIP for binding to the target molecular structure; and
converting at least a portion of said MIP to a functional group for forming a covalent bond with the target molecular structure upon binding of said MIP to the activated target molecular structure. - View Dependent Claims (48, 49, 50, 51, 52, 53, 54)
-
Specification