Composite material for biological or biochemical analysis microfluidic system

• US 20040126779A1
• Filed: 10/20/2003
• Published: 07/01/2004
• Est. Priority Date: 02/21/2002
• Status: Active Grant

• Current Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Original Assignee: Commissariat a L'Energie Atomique (French Alternative Energies and Atomic Energy Commission)
• Inventors: Revol-Cavalier, Frederic, Combette, Philippe, Beneyton, Bernard, Mittler, Frederique
• Granted Patent: US 7,214,478 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G01N 33/552 Glass or silica

Abstract

The invention concerns a component for biological or biochemical analysis microsystems formed from a support and having at least one chemically functionalised surface zone, in order to allow in said zone the formation of a chemistry for anchoring biological or biochemical elements, and/or electrically, in order to allow in said zone the formation of electrical charges. The support comprises at least one part (21) formed of a composite material, said composite material being a mixture of at least one inert material and at least one chemically and/or electrically functionalisable material to provide said functionalised surface zone.

Claims

1. Component for biological or biochemical analysis microsystems formed from a support and having at least one chemically functionalised surface zone, in order to allow in said zone the formation of a chemistry for anchoring biological or biochemical elements, and/or electrically, in order to allow in said zone the formation of electrical charges, characterised in that said support comprises at least one part (16, 21, 33, 46) formed of a composite material, said composite being a mixture of at least one inert material and at least one chemically and/or electrically functionalisable material to provide said functionalised surface zone.

2. Component according to claim 1, characterised in that the inert material of the composite material is a material chosen from among a polymer, a plastic, a resin and an adhesive.

3. Component according to claim 2, characterised in that the polymer is a polyimide, a poly (dimethylsiloxane) or an epoxy type photosensitive resin.

4. Component according to claim 1, characterised in that said part (16) forms the support in its entirety.

5. Component according to claim 1, characterised in that the support comprises a substrate (20, 30) supporting said part.

6. Component according to claim 5, characterised in that the substrate (20, 30) is in a material chosen from among glass, silica, silicon, a polymer and a metal.

7. Component according to any of claims 1 to 6, characterised in that said part (21) is structured.

8. Component according to any of claims 1 to 7, characterised in that the functionalisable material is chosen from among silica, synthesised silica, silicon nitride, a metal and a functionalisable polymer.

9. Component according to any of claims 1 to 8, characterised in that the composite material is a mixture comprising a phase of inert material and a phase of functionalisable material.

10. Component according to any of claims 1 to 8, characterised in that the composite material is formed of elements of a functionalisable material dispersed in the inert material.

11. Component according to claim 10, characterised in that the functionalisable material is in the form of beads (42).

12. Component according to any of claims 1 to 11, characterised in that said surface zone supports chemical functions suited to assuring the attachment of biological elements or other chemical functions on said surface zone.

13. Component according to any of claims 1 to 11, characterised in that said surface zone supports chemical functions suited to assuring the presence of electrical charges on said surface zone.

14. Method for producing a component for biological or biochemical analysis microsystems from a support, said support needing to have at least one chemically functionalised surface zone to allow in said zone the formation of a chemistry for anchoring biological or biochemical elements, and/or electrically, to allow in said zone the formation of electrical charges, characterised in that it comprises a support comprising at least one part (16, 21, 33, 46) in composite material, said composite material being a mixture of at least one inert material and at least one chemically and/or electrically functionalisable material in order to provide said functionalised surface zone.

15. Method according to claim 14, characterised in that the composite material is obtained by mixing in liquid phases the inert material and the functionalisable material, the mixture then being solidified in order to provide said part in composite material.

16. Method according to claim 14, characterised in that the composite material is obtained by dispersion of elements in functionalisable material in the inert material in liquid phase, the mixture then being solidified in order to provide said part in composite material.

17. Method according to claim 16, characterised in that said elements in functionalisable material are in the form of beads (42).

18. Method according to one of claims 16 or 17, characterised in that the inert material in liquid phase in which are dispersed said elements is poured onto a support with impression(s) (10) before being solidified.

19. Method according to claim 18, characterised in that the support with impression(s) (10) is removed after solidification of the mixture.

20. Method according to one of claims 16 or 17, characterised in that the inert material in liquid phase in which are dispersed said elements is deposited on a support (20, 30) before being solidified.

21. Method according to claim 20, characterised in that the inert material being a photosensitive material, said part in composite material (21) is, after solidification, structured by photo-lithography.

22. Method according to claim 20, characterised in that the deposition is made on one surface of the support (30) having at least one impression (31), the composite material (32) is, after solidification, eliminated outside of the impression.

23. Method according to claim 22, characterised in that the inert material being a photosensitive material, the elimination of the composite material outside of the impression is achieved by photo-lithography.

24. Method according to claim 14, characterised in that the support (40) has one face with at least one impression (41), elements in functionalisable material (42) are deposited at the base of the impression, then the inert material (43) in liquid phase is poured onto said face of the support, then the inert material is solidified to provide the composite material (46) at the base of the impression, the support being finally removed.

25. Method according to claim 24, characterised in that said elements in functionalisable material (42) are in the form of beads.

26. Method according to any of claims 15 to 25, characterised in that said solidification is obtained by a thermal treatment.