High affinity peptide- containing nanoparticles
First Claim
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1. A water-soluble polymeric nanoparticle functionalized by at least two peptide moieties covalently linked to the nanoparticle polymeric core structure, the peptide moieties possessing high affinity to biomolecules.
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Abstract
The present invention is directed to polymeric nanoparticles functionalized with two or more peptide moieties that possess high affinity to biomolecular targets, the peptide moieties being covalently linked to the nanoparticle polymeric core structure, either directly or via a linker molecule. The invention is further directed to methods of synthesizing these polymeric nanoparticles and to the various applications for which they may be used.
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Citations
26 Claims
- 1. A water-soluble polymeric nanoparticle functionalized by at least two peptide moieties covalently linked to the nanoparticle polymeric core structure, the peptide moieties possessing high affinity to biomolecules.
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5. A water-soluble polymeric nanoparticle comprising i) at least two peptide moieties covalently linked to the nanoparticle polymeric core structure, each of the peptide moieties possessing high affinity to a therapeutic protein;
- and ii) a therapeutic protein noncovalently linked to the peptide moiety and being at least partially enclosed within the nanoparticle.
- View Dependent Claims (6)
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21. A method for the molecular recognition of a biomolecular target, the method comprising exposing the biomolecular target to a water-soluble polymeric nanoparticle functionalized by at least two peptide moieties covalently linked to the nanoparticle polymeric core structure, at least one of the peptide moieties possessing high affinity to proteins expressed on the biomolecular target.
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22. A method for controllably releasing a therapeutic protein to an environment in a mammalian body, the method comprising administering to the environment a water-soluble polymeric nanoparticle comprising i) at least two peptide moieties covalently linked to the nanoparticle polymeric core structure, each of the peptide moieties possessing high affinity to the therapeutic protein;
- and ii) the therapeutic protein noncovalently linked to the peptide moiety and being at least partially enclosed within the nanoparticle.
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23. A method for the controlled delivery of a therapeutic protein to the vicinity of a targeted cell or tissue type, the method comprising administering to an environment containing the targeted cell or tissue type, a water-soluble polymeric nanoparticle comprising i) at least two first peptide moieties covalently linked to the nanoparticle polymeric core structure, each of the first peptide moieties possessing high affinity to the targeted cell or tissue type;
- ii) at least two second peptide moieties covalently linked to the nanoparticle polymeric core structure, each of the second peptide moieties possessing high affinity to the therapeutic protein; and
iii) the therapeutic protein noncovalently linked to the second peptide moiety and being at least partially enclosed within the nanoparticle.
- ii) at least two second peptide moieties covalently linked to the nanoparticle polymeric core structure, each of the second peptide moieties possessing high affinity to the therapeutic protein; and
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24. A method for synthesizing a water-soluble peptide-functionalized polymeric nanoparticle, the method comprising adding an aqueous phase containing hydrophilic building blocks, the building blocks comprising hydrophilic monomers with crosslinkable groups, to an organic solvent comprising at least one surfactant;
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reacting the crosslinkable groups of the building blocks to covalently crosslink the building blocks to give a hydrophilic polymeric nanoparticle;
removing surfactant and the organic solvent;
adding peptide moieties to a solution containing the nanoparticle, the peptide moieties comprising a functionality for attachment to the nanoparticle and the peptide moieties possessing high affinity to proteins expressed on a cell or a tissue; and
reacting the peptide moieties and the nanoparticle to covalently bond the peptide and nanoparticle. - View Dependent Claims (25, 26)
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Specification