Apparatus for transfer of an array of liquids and methods for manufacturing same

• US 20040129676A1
• Filed: 01/07/2003
• Published: 07/08/2004
• Est. Priority Date: 01/07/2003
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Tan, Roy H.
• Application Number:
  US10/337,834
• Publication Number:
  US 20040129676A1
• Time in Patent Office: Days
• US Class Current: 216/80
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00371 comprising electrodes
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2400/025 tapping tip on substrate
• CPC Class Codes: B01L 2400/027 electrostatic forces between substrate and tip
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: H01J 49/04 Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

A method for producing an apparatus for transferring small amounts of liquids includes bonding a plurality of parallel fibers having plural coaxial layers into a bundle, slicing the bundle of parallel fibers in planes perpendicular to the direction of the fibers to form two opposite, planar surfaces, and selectively etching the fiber layers to create etched wells in the fibers at one of the planar surfaces. The etched wells are in fluid communication with corresponding capillary nozzles of the fibers that extend to an opposite one of the planar surfaces. Various apparatus configurations of the present invention include liquid transfer devices manufactured utilizing one or more of the various method configurations of the present invention. By way of example only, a bundle of three-layer optical fibers or a bundle of hollow two-layer optical fibers may be utilized to produce a liquid transfer device.

Claims

1. An apparatus for transfer of an array of liquids, said apparatus comprising a bonded array of parallel capillary tubes, the array having a planar well side and an opposite, planar nozzle side, wherein a plurality of said capillary tubes include a microwell at the planar well side and a capillary nozzle in fluid communication with the microwell extending to the planar nozzle side.

2. An apparatus in accordance with claim 1 wherein said capillary tubes comprise optical fiber.

3. An apparatus in accordance with claim 1 wherein said microwells are configured to hold a volume of about 5 microliters of liquid.

4. An apparatus in accordance with claim 1 wherein said capillary nozzles comprise an annular tip with a hole passing therethrough at the planar nozzle side of said apparatus.

5. An apparatus in accordance with claim 4 wherein ends of said cylindrical tips are flush with a plane of the planar nozzle side of said apparatus.

6. An apparatus in accordance with claim 1 wherein said capillary tubes are about 300 microns in length, including a well configured to hold about 5 microliters liters of liquid, and the capillary nozzle has an opening of between about 1 and about 10 microns in diameter.

7. An apparatus in accordance with claim 6 including at least 96 capillary tubes.

8. An apparatus in accordance with claim 7 including between 96 and 1536 capillary tubes within an area of no more than about 21 square millimeters.

9. An apparatus in accordance with claim 1 further comprising a hydrophobic insulation between wells deposited on a surface land area of said planar well side.

10. An apparatus in accordance with claim 9 wherein the hydrophobic insulation comprises at least one member of the group consisting of deposited silica, Teflon, or fluorocarbon material.

11. An apparatus in accordance with claim 1 wherein the planar well side and the planar nozzle side are polished to an optical flatness.

12. A method for making a liquid transfer device, said method comprising:

bonding a plurality of parallel fibers having plural coaxial layers into a bundle;

slicing the bundle of parallel fibers in planes perpendicular to the direction of the fibers to form two opposite, planar surfaces,

selectively etching the fiber layers to create etched wells in the fibers at one of the planar surfaces, wherein the etched wells are in fluid communication with corresponding capillary nozzles of the fibers that extend to an opposite one of the planar surfaces.

13. A method in accordance with claim 12 wherein said bonding a plurality of parallel fibers having plural coaxial layers into a bundle comprises bonding a plurality of parallel optical fibers having coaxial layers into a bundle.

14. A method in accordance with claim 12, wherein the parallel fibers are three-layer fibers, each layer corresponding to one of said coaxial layers;

and further wherein

said selectively etching the fiber layers comprises etching center layers of said coaxial layers between one said planar surface and said opposite planar surface to form said capillary nozzles, and etching a well in middle layers of said coaxial layers surrounding said center layers from one said planar surface only a portion of the distance to the opposite planar surface to form said etched wells.

15. A method in accordance with claim 14 wherein said selectively etching the fiber layers further comprises etching said nozzles to a diameter in a range of about 1 micron to about 10 microns.

16. A method in accordance with claim 15 wherein said selectively etching the fiber layers further comprises etching said wells to a volume of about 5 microliters each.

17. A method in accordance with claim 14 further comprising depositing a hydrophobic insulation between wells on land areas of the planar surface having the etched wells.

18. A method in accordance with claim 17 wherein said depositing a hydrophobic insulation comprises depositing at least one member of the group consisting of silica, Teflon, and fluorocarbon material between wells on the land areas of the planar surface having the etched wells.

19. A method in accordance with claim 12 further comprising pulling the bundle of parallel fibers into a desired dimension.

20. A method in accordance with claim 12 further comprising polishing the planar surfaces to an optical flatness.

21. A method in accordance with claim 12 further comprising applying a resist material around the capillary nozzle openings on one of the planar surfaces and etching a portion of the planar surface around the resist materials to thereby form tips around the nozzle openings.

22. A method in accordance with claim 12 wherein said bonding a plurality of parallel fibers having plural coaxial layers into a bundle comprises bonding a plurality of hollow fibers into a bundle.

23. A method in accordance with claim 22 wherein said coaxial layers surround capillary holes through the fibers, and said selectively etching the fiber layers comprises etching a layer surrounding the capillary hole only a portion of the distance to the opposite planar surface to form said etched wells.

24. A method in accordance with claim 23 wherein said capillary holes have a diameter in a range of about 1 micron to about 10 microns, and said selectively etching the fiber layers further comprises etching said wells to a volume of about 5 microliters each.

25. A method in accordance with claim 23 further comprising depositing a hydrophobic insulation between wells on land areas of the planar surface having the etched walls.

26. A method in accordance with claim 25 wherein said depositing a hydrophobic insulation comprises depositing silica between wells on the land areas of the planar surface having the etched wells.

27. A method in accordance with claim 22 further comprising pulling the bundle of parallel fibers into a desired dimension.

28. A method in accordance with claim 22 further comprising polishing the planar surfaces to an optical flatness.

29. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 12.

30. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 13.

31. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 14.

32. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 15.

33. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 21.

34. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 22.

35. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 23.

36. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 24.

37. An apparatus for transfer of an array of liquids produced by a method in accordance with claim 25.

38. A method in accordance with claim 12 wherein said bonding a plurality of parallel fibers comprises bonding a plurality of hollow three-layer fibers, and selectively etching the fiber layers comprises applying different etchants to the opposite planar surfaces.

39. A method in accordance with claim 12 wherein said bonding a plurality of parallel fibers comprises bonding a plurality of hollow three-layer fibers, and selectively etching the fiber layers comprises applying no more than two different etchants, wherein one said etchant is applied to one of the opposite planar surfaces and the other said etchant to the other one of the opposite planar surfaces.

40. A method in accordance with claim 12 wherein the parallel fibers are hollow fibers with capillary voids, and said method further comprises temporarily filling the capillary voids.

41. A method in accordance with claim 40 wherein said capillary voids are temporarily filled with wax.

42. A method in accordance with claim 12 wherein said bonding a plurality of parallel fibers comprises bonding a plurality of four layer fibers.

43. A method in accordance with claim 12 wherein said selectively etching the fiber layers comprises etching out undoped silicon and silica utilizing a mixture of potassium hydroxide, water, and isopropyl alcohol.

44. A method in accordance with claim 43 further comprising an additional etching utilizing a buffered acid solution.

45. A method in accordance with claim 44 wherein the buffered acid solution comprises a mixture of hydrofluoric acid, nitric acid, and acetic acid.