Modified oilseed material

• US 20040131747A1
• Filed: 10/30/2003
• Published: 07/08/2004
• Est. Priority Date: 06/18/2001
• Status: Active Grant

• Current Assignee: Cargill, Inc. (Agricultural Services Division)
• Original Assignee: Cargill, Inc. (Agricultural Services Division)
• Inventors: Martinson, Wade S, Friedrich, Jane E, Bjork, Roger E, Satyavolu, Jagannadh V, Inman, Thomas C, Sperber, William H, Stark, Ann M, Smedley, Troy R, Muralidhara, Harapanahalli S, Pemble, Trent H, Porter, Michael A, Kellerman, James C, Johnson, Scott D, Foster, William G, Karleskind, Daniele, Purtle, Ian
• Granted Patent: US 7,429,399 B2
• Time in Patent Office: Days
• US Class Current: 426/614
• CPC Class Codes: A23G 1/305 Products for covering, coating, finishing, decorating
• CPC Class Codes: A23G 2200/00 containing organic compounds, e.g. synthetic flavouring agents
• CPC Class Codes: A23G 2200/08 containing cocoa fat if specifically mentioned or containing products of cocoa fat or containing other fats, e.g. fatty acid, fatty alcohol, their esters, lecithin, paraffins
• CPC Class Codes: A23G 2200/10 containing amino-acids, proteins, e.g. gelatine, peptides, polypeptides
• CPC Class Codes: A23G 3/343 Products for covering, coating, finishing, decorating
• CPC Class Codes: A23G 3/346 Finished or semi-finished products in the form of powders, paste or liquids A23G3/343 takes precedence
• CPC Class Codes: A23G 3/40 characterised by the fats used containing dairy products A23G3/46
• CPC Class Codes: A23G 3/54 Composite products, e.g. layered, coated, filled
• CPC Class Codes: A23G 9/322 Products for covering, coating, finishing, decorating
• CPC Class Codes: A23G 9/327 characterised by the fatty product used, e.g. fat, fatty acid, fatty alcohol, their esters, lecithin, glycerides butter, dairy cream or milkfat A23G9/40
• CPC Class Codes: A23G 9/48 Composite products, e.g. layered , laminated, coated, filled
• CPC Class Codes: A23G 9/52 Liquid products; Solid products in the form of powders, flakes or granules for making liquid products ; Finished or semi-finished solid products, frozen granules
• CPC Class Codes: A23J 1/14 from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
• CPC Class Codes: A23L 11/07 Soya beans, e.g. oil-extracted soya bean flakes A23L11/30 takes precedence
• CPC Class Codes: A23L 13/426 Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols A23L13/422 takes precedence
• CPC Class Codes: A23L 2/66 Proteins
• CPC Class Codes: A23L 33/185 Vegetable proteins
• CPC Class Codes: A23L 7/126 Snacks or the like obtained by binding, shaping or compacting together cereal grains or cereal pieces, e.g. cereal bars
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/032 Citric acid
• CPC Class Codes: A23V 2250/5062 : Inulin
• CPC Class Codes: A23V 2250/5072 : Pectine, pectinate
• CPC Class Codes: A23V 2250/5488 : Soybean protein
• CPC Class Codes: A23V 2250/606 : Fructose
• CPC Class Codes: A23V 2250/636 : Trehalose
• CPC Class Codes: A23V 2250/702 : Vitamin A
• CPC Class Codes: A23V 2250/708 : Vitamin C
• CPC Class Codes: A23V 2300/34 : Membrane process

Abstract

A modified oilseed material formed from oilseed-based material is described. The modified oilseed material can be utilized in a variety of nutritional applications, including the preparation of protein supplemented food products such as beverages, processed meats, frozen desserts, confectionery products, dairy-type products, and cereal grain products. The modified oilseed material typically includes at least 85 wt. % protein (dry solids basis), at least about 40 wt % of the protein in the modified oilseed material has an apparent molecular weight of at least 300 kDa, and/or the modified oilseed material has a MW50 of at least about 200 kDa.

Claims

1. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa; and

at least about 40 wt. % of the protein in a 50 mg sample of the modified oilseed material is soluble in 1.0 mL water at 25°

C.

2. The modified oilseed material of claim 1 having an EOR of no more than about 0.75 mL.

3. The modified oilseed material of claim 1 wherein a 13.5% aqueous solution of the modified oilseed material forms a gel having a breaking strength of no more than about 25 g.

4. The modified oilseed material of claim 1 having a viscosity slope of at least about 20 cP/min.

5. The modified oilseed material of claim 1 having a melting temperature of at least about 87°

C. and has a bacteria load of no more than about 50,000 cfu/g.

6. The modified oilseed material of claim 1 having an MW₅₀ of at least about 400 kDa.

7. The modified oilseed material of claim 1 having a turbidity factor of no more than about 0.95.

8. The modified oilseed material of claim 1 having a dry Gardner L value of at least about 85.

9. The modified oilseed material of claim 1 having an NSI of at least about 80.

10. The modified oilseed material of claim 1 including at least about 1.4 wt. % cysteine as a percentage of total protein.

11. The modified oilseed material of claim 1 having a ratio of sodium ions to a total amount of sodium, calcium and potassium ions of no more than about 0.5.

12. The modified oilseed material of claim 1 having no more than about 7000 mg/kg (dsb) sodium ions.

13. The modified oilseed material of claim 1 wherein the modified oilseed material is a modified soybean material including at least about 90 wt. % (dsb) protein.

14. The modified oilseed material of claim 1 having a substantially bland taste.

15. The modified oilseed material of claim 1 comprising a flavor component content including no more than about 500 ppb benzaldehyde;

no more than about 2500 ppb 2-pentyl furan;

no more than about 600 ppb 2-heptanone; and

no more than about 200 ppb E,E-2,4-decadienal.

16. The modified oilseed material of claim 1 comprising a flavor component content which includes no more than about 350 ppb benzaldehyde;

no more than about 450 ppb 2-heptanone;

no more than about 150 ppb E,E-2,4-decadienal; and

no more than about 50 ppb E,E-2,4-nonadienal.

17. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa; and

the modified oilseed material has a viscosity slope of at least about 20.

18. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa; and

the modified oilseed material has a melting temperature of at least 87 C.

19. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa; and

the modified oilseed material has an EOR of no more than about 0.75 mL.

20. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa; and

the modified oilseed material has a turbidity factor of no more than about 0.95 at 500 nm.

21. A modified oilseed material comprising at least about 85 wt. % (dsb) protein;

wherein said modified oilseed material has a bacterial load of no more than 50,000 cfu/g and a melting temperature of at least 8.7°

C.; and

at least about 40 wt. % of the protein has an apparent molecular weight of greater than 300 kDa.

22. A method for producing a modified oilseed material comprising:

extracting oilseed material with an aqueous solution to form a suspension of particulate matter in an oilseed extract;

passing the extract through a filtration system including a microporous membrane to produce a first permeate and a protein-enriched retentate, wherein the microporous membrane has a filtering surface with a contact angle of no more than 30 degrees.

23. The method of claim 22 comprising passing the extract through the filtration system under a transmembrane pressure of no more than 50 psig.

24. The method of claim 22 comprising passing the extract through the filtration system at 55°

C. to 60°

C.

25. The method of claim 22 further comprising diafiltering the protein-enriched retentate through the filtration system to produce a diafiltration retentate and a diafiltration permeate.

26. The method of claim 22 wherein the aqueous solution has a pH of 6.5 to 10.0.

27. The method of claim 22 wherein passing the extract through the filtration system comprises first passing an original volume of the extract through the filtration system while adding water to the extract in a feed tank so as to substantially maintain the original volume, and second passing the extract through the filtration system while allowing the retentate to be concentrated by a factor of at least 2.5 relative to the original volume.

28. The method of claim 22 further comprising heating the retentate to at least 75°

C. for sufficient time to form a pasteurized retentate.

29. The method of claim 22 comprising extracting the oilseed material in a multistage operation which includes extracting the oilseed material in an initial extraction stage with an aqueous solution having a pH of 6.5 to 7.5 and extracting the oilseed material in a final extraction stage with an aqueous solution having a pH of 8.0 to 9.0.

30. The method of claim 22 comprising extracting the oilseed material in a multistage countercurrent operation which includes extracting the oilseed material at a pH of 6.5 to 7.5 in an initial extraction stage with a protein-rich liquor stream from a subsequent extraction stage;

and extracting the oilseed material at a pH of 8.0 to 9.0 in a final extraction stage with an aqueous sodium hydroxide solution.

31. The method of claim 22 comprising extracting the oilseed material in a multistage countercurrent operation which includes heating a protein-rich extract from a selected stage to at least about 75°

C. to form a heat-treated extract; and

extracting oilseed material with the heat-treated extract in a different extraction stage.

32. The method of claim 31 wherein heating the protein-rich extract from the initial stage comprises heating a slurry of partially extracted oilseed material and the protein-rich extract.

33. The method of claim 22 wherein the microporous membrane is an ultrafiltration membrane having an MWCO of about 25,000 to 500,000.

34. The method of claim 22 wherein the microporous membrane has a pore size of 0.1μ

to 1.5μ

.

35. The method of claim 22 wherein the membrane is a modified polyacrylonitrile membrane.

36. The method of claim 22 wherein the membrane is designed for exposure to temperatures up to at least about 75°

C.;

pHs ranging from about 2 to 11; and

is capable of withstanding treatment with an oxidizing solution.

37. The modified oilseed material of claim 22 comprising extracting the oilseed material with the aqueous solution for no more than about 10 minutes.

38. The modified oilseed material of claim 22 wherein the process is a continuous, multistage process with an apparent contact time of no more than 20 minutes.

39. The method of claim 22 comprising extracting soybean material at about 20°

C. to 60°

C. with an aqueous solution having a pH of 6.5 to 10 to form a mixture of particulate matter in an extract solution;

removing at least a portion of the particulate matter from the mixture to form a clarified extract; and

passing the clarified extract at 55°

C. to 60°

C. through the filtration system.

40. A modified oilseed material produced by a process which includes extracting oilseed material with an aqueous solution to form a suspension of particulate matter in an oilseed extract;

and passing the extract through a filtration system including a microporous membrane to produce a permeate and a protein-enriched retentate, wherein the microporous membrane has a filtering surface with a contact angle of no more than about 30 degrees.

41. The modified oilseed material of claim 40 wherein the process comprises extracting oilseed material at about 20°

C. to 75°

C. with an aqueous solution having a pH of 6.5 to 10.0 to form a mixture of particulate matter in an extract solution;

removing at least a portion of the particulate matter from the mixture to form a clarified extract; and

passing the clarified extract at about 55°

C. to 60°

C. through the filtration system, wherein the microporous membrane includes modified polyacrylonitrile.

42. A food composition comprising a modified oilseed material, wherein the modified oilseed material comprises at least 85 wt. % protein on a dry solids basis;

at least about 40 wt. % of the protein has an apparent molecular weight of at least 300 kDa; and

at least about 40 wt. % of the protein in a 50 mg sample of the modified oilseed material is soluble in 1.0 mL water at 25°

C.

43. The food composition of claim 42 wherein said food composition is a pasteurized food composition.

44. The food composition of claim 42 wherein said food composition is a beverage composition, a processed meat composition, a confectionery composition, or a frozen dessert composition.