DNA chip and its preparation

• US 20040132081A1
• Filed: 12/16/2003
• Published: 07/08/2004
• Est. Priority Date: 02/08/1999
• Status: Abandoned Application

• Current Assignee: Kosuke Tashiro, Masashi Hakamata, Satoru Kuhara, Shigeru Muta, Tohru Tsuchiya
• Original Assignee: Kosuke Tashiro, Masashi Hakamata, Satoru Kuhara, Shigeru Muta, Tohru Tsuchiya
• Inventors: Hakamata, Masashi, Tashiro, Kosuke, Muta, Shigeru, Tsuchiya, Tohru, Kuhara, Satoru
• Application Number:
  US10/737,328
• Publication Number:
  US 20040132081A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2527/125 : Specific component of sample, medium or buffer
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

A DNA chip favorably employable for detecting a DNA fragment complementary to the oligo- or polynucleotide fixed to the chip is composed of a solid carrier and oligonucleotide or polynucleotide which is fixed to the carrier in the presence of a hydrophilic polymer.

Claims

1. A DNA chip comprising a solid carrier and oligonucleotide or polynucleotide which is fixed to the carrier in the presence of a hydrophilic polymer.

2. A DNA chip of claim 1, wherein the oligonucleotide or polynucleotide is fixed to the carrier at its one end portion.

3. The DNA chip of claim 1, wherein the solid carrier is coated with poly-L-lysine.

4. The DNA chip of claim 1, wherein the oligonucleotide or polynucleotide has a NH₂ terminal and is fixed to the carrier at its NH₂ terminal.

5. The DNA chip of claim 3, wherein the oligonucleotide or polynucleotide has a NH₂ terminal and is fixed to the carrier at its NH₂ terminal.

6. The DNA chip of claim 1, wherein the hydrophilic polymer is selected from the group consisting of poly-(1,4-diazoniabicyclo[2.2.2]octane-1,4-diylmethylene-1,4-phenylenemethylene chloride), polyacrylamide, polyethylene glycol, poly(sodium acrylate), carboxymethylcellulose and albumin.

7. The DNA chip of claim 1, wherein the oligonucleotide or polynucleotide is known in its base sequence.

8. The DNA chip of claim 1, wherein the oligonucleotide or polynucleotide is a synthetically prepared product.

9. The DNA chip of claim 1, wherein the oligonucleotide or polynucleotide is a cleaved DNA fragment.

10. A method of fixing an oligonucleotide or polynucleotide to a solid carrier which comprises spotting an aqueous solution containing the oligonucleotide or polynucleotide and a hydrophilic polymer onto the carrier.

11. The method of claim 10, wherein the oligonucleotide or polynucleotide is fixed to a solid carrier at its one end portion.

12. The method of claim 10, which further comprises the steps of washing the spotted carrier and drying the washed carrier.

13. A process for detecting a DNA fragment complementary to oligonucleotide or polynucleotide fixed to a DNA chip comprising the steps of spotting an aqueous solution containing the DNA fragment labelled with a fluorescent moiety on the DNA chip which comprises a solid carrier and oligonucleotide or polynucleotide which is fixed to the carrier in the presence of a hydrophilic polymer, incubating the spotted chip for performing hybridization between the oligonucleotide or polynucleotide and the complementary DNA fragment in the aqueous solution, and detecting the hybridized complementary fragment by fluorometry.