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Determining the density of functional moieties on polymer reagents

  • US 20040132092A1
  • Filed: 01/03/2003
  • Published: 07/08/2004
  • Est. Priority Date: 01/03/2003
  • Status: Abandoned Application
First Claim
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1. A method of determining the density of functional moieties on polymer substrates comprising:

  • (a) attaching a dye molecule to a polymer substrate, said dye molecule absorbing infrared radiation at a wavelength of at least 600 nm;

    (b) attaching a compound as a functional moiety to said polymer substrate with attached dye molecules, said compound being selected to react with an analyte in a biological sample;

    (c) attaching the dye molecule selected in (a) as a label to a second sample of said polymer substrate and determining by their absorption of infrared radiation the number of dye molecules attached to said polymer substrate;

    (d) determining the absorption of ultraviolet radiation by the functional moieties and by infrared radiation absorption of dye molecules in the polymer substrate of (b); and

    (e) determining the number of functional moieties on said polymer substrate of (b) relative to the number of attached dye molecules determined in (c), said number of functional moieties representing the density of said functional molecules.

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