Label-free high-throughput optical technique for detecting biomolecular interactions
First Claim
Patent Images
1. An optical device comprising:
- (a) a two-dimensional grating comprised of a material having a high refractive index; and
(b) a substrate layer that supports the two-dimensional grating;
wherein, when the optical device is illuminated a resonant grating effect is produced on the reflected radiation spectrum, and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect.
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Abstract
Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.
299 Citations
44 Claims
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1. An optical device comprising:
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(a) a two-dimensional grating comprised of a material having a high refractive index; and
(b) a substrate layer that supports the two-dimensional grating;
wherein, when the optical device is illuminated a resonant grating effect is produced on the reflected radiation spectrum, and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect. - View Dependent Claims (2, 3, 4, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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- 5. An optical device comprising a substrate and a two-dimensional grating wherein the substrate and the two-dimensional grating comprise a single unit, wherein the surface of the single unit comprising the two-dimensional grating is coated with a material having a high refractive index.
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18. A biosensor comprising:
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(a) a two-dimensional grating comprised of a material having a high refractive index, wherein the two-dimensional grating is comprised of a repeating pattern of shapes selected from the group consisting of lines, squares, circles, ellipses, triangles, trapezoids, sinusoidal waves, ovals, rectangles, and hexagons;
(b) a substrate layer that supports the two-dimensional grating; and
(c) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate layer;
wherein, when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum, and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
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26. An optical device comprising:
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(a) a sheet material having a first and second surface, wherein the first surface defines relief volume diffraction structures; and
(b) a reflective material coated onto the first surface of the sheet material;
wherein the optical device is capable of reflecting light predominantly at a first single optical wavelength when illuminated with a broad band of optical wavelengths as a result of optical interference. - View Dependent Claims (27, 28)
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29. A detection system comprising:
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(a) a biosensor comprising;
(i) a sheet material having a first and second surface, wherein the first surface defines relief volume diffraction structures;
(ii) a reflective material coated onto the first surface of the sheet material; and
(iii) one or more specific binding substances immobilized on the reflective material;
wherein the biosensor reflects light predominantly at a first single optical wavelength when illuminated with a broad band of optical wavelengths, and wherein the biosensor reflects light at a second single optical wavelength when the one or more specific binding substances are immobilized on the reflective surface, wherein the reflection at the second optical wavelength of light results from optical interference; and
(c) a light source that directs light to the reflective surface of the biosensor; and
(d) a detector that detects light reflected from the reflective surface of the biosensor.
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- 30. A resonant reflection structure comprising a two-dimensional grating having a pattern of concentric rings, wherein the difference between an inside diameter and an outside diameter of each concentric ring is equal to about one-half of a grating period, wherein each successive ring has an inside diameter that is about one grating period greater than an inside diameter of a previous ring wherein when the structure is illuminated with an illuminating light beam, a reflected radiation spectrum is produced that is independent of an illumination polarization angle of the illuminating light beam.
- 33. A transmission filter structure comprising a two-dimensional grating arranged in a pattern of concentric rings, wherein the difference between an inside diameter and an outside diameter is equal to about one-half of a grating period, wherein each successive concentric ring has an inside diameter that is about one grating period greater than an inside diameter of a previous ring, wherein when the structure is illuminated with an illuminating light beam, a transmitted radiation spectrum is produced that is independent of an illumination polarization angle of the illuminating light beam.
- 36. A resonant reflection structure comprising an array of holes or posts arranged such that the holes or posts are centered on corners and in the center of hexagons, wherein the hexagons are arranged in a closely packed array, wherein when the structure is illuminated with an illuminating light beam, a reflected radiation spectrum is produced that is independent of an illumination polarization angle of the illuminating light beam.
- 39. A transmission filter structure comprising an array of holes or posts arranged such that the holes or posts are centered on corners and in the center of hexagons, wherein the hexagons are arranged in a closely packed array, wherein when the structure is illuminated with an illuminating light beam, a transmitted radiation spectrum is produced that is independent of an illumination polarization angle of the illuminating light beam.
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42. An optical device comprising:
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(a) a first two-dimensional grating comprising a high refractive index material and having a top surface and a bottom surface; and
(b) a second two-dimensional grating comprising a high refractive index material and having a top surface and a bottom surface, wherein the top surface of the second two-dimensional grating is attached to the bottom surface of the first two-dimensional grating;
wherein, when the biosensor is illuminated two resonant grating effects are produced on the reflected radiation spectrum, and wherein the depth and period of both of the two-dimensional gratings are less than the wavelength of the resonant grating effects. - View Dependent Claims (43)
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44. An optical device comprising:
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(a) a first two-dimensional grating comprising a high refractive index material and having a top surface and a bottom surface;
(b) a substrate layer comprising a top surface and a bottom surface, wherein the top surface of the substrate supports the bottom surface of the first two-dimensional grating; and
(c) a second two-dimensional grating comprising a high refractive index material and having a top surface and a bottom surface, wherein the bottom surface of the second two-dimensional grating is attached to the bottom surface of the substrate;
wherein, when the biosensor is illuminated two resonant grating effects are produced on the reflected radiation spectrum, and wherein the depth and period of both of the two-dimensional gratings are less than the wavelength of the resonant grating effects.
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Specification