Method for improving a reading resolution of a biosensor

• US 20040132203A1
• Filed: 12/19/2003
• Published: 07/08/2004
• Est. Priority Date: 12/31/2002
• Status: Active Grant

• Current Assignee: Intellectual Ventures I LLC (Intellectual Ventures LLC)
• Original Assignee: Hiyes International Co., Ltd.
• Inventors: Huang, Yin-Chun, Wang, Kuo-Jeng
• Granted Patent: US 7,510,642 B2
• Time in Patent Office: Days
• US Class Current: 436/149
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875

Abstract

A method for improving a reading resolution of a biosensor is provided. The present invention includes applying a specimen on a chip of a biosensor, and a voltage-time discharge curve is established in response to a specific component in the specimen detected by the chip. Using a voltage V₀ of a sampling time t₀ of the voltage-time discharge curve as a central voltage, and selecting a plurality of respective voltages of sampling times close to the sampling time t₀. Obtaining an average voltage of the central voltage V₀ and these selected respective voltages to serve as an output voltage of the sampling time t₀. Determining a respective average voltage corresponding to each sampling time of the voltage-time discharge curve before the time of discharge ending based on the former step, to serve as the output voltage of the each sampling time. The each output voltage is converted to a set of digital signals. A reading of the concentration of the specific component in the specimen is determined in accordance with these digital signals.

Claims

1. A method for improving a reading resolution of a biosensor, comprising:

providing a specimen on a chip of a biosensor, said chip generating a time-varying response current in response to a specific component of said specimen, so that a voltage-time discharge curve is established in response to a specific component of said specimen;

using a voltage V₀ of a sampling time t₀ of said voltage-time discharge curve as a central voltage, and selecting a plurality of respective voltages whose sampling times close to said sampling time t₀, obtaining an average voltage of said central voltage V₀ and said respective voltages to serve as an output voltage corresponding to said sampling time t₀;

obtaining an average voltage corresponding to each said sampling time of said voltage-time discharge curve before the time of discharge ending to serve as an output voltage of each said sampling time based on the former step;

converting said output voltage of each said sampling time of said voltage-time discharge curve to a set of binary digital signals; and

determining a reading of a concentration of said specific component in accordance with the sets of binary digital signals before the time of discharge ending.

2. The method of claim 1, wherein the voltage of each said sampling time of said voltage-time discharge curve is an integer.

3. The method of claim 1, wherein said respective voltages close to said central voltage V₀ includes three voltages V₁, V₂ and V₃ close to said central voltage V₀ in said voltage-time discharge curve.

4. The method of claim 3, wherein said three voltages V₁, V₂ and V₃ includes two voltages prior to said central voltage V₀ and one voltage behind said central voltages V₀ in said voltage-time discharge curve.

5. The method of claim 3, wherein said three voltages includes one voltage prior to said central voltage V₀ and two voltages behind said central voltage V₀ in said voltage-time discharge curve.

6. The method of claim 3, wherein said three voltages are prior to said central voltages V₀ in said voltage-time discharge curve.

7. The method of claim 3, wherein said three voltages are behind said central voltages V₀ in said voltage-time discharge curve.

8. The method of claim 1, wherein the sampling interval is in the range of millisecond.

9. The method of claim 1, wherein the sampling time is in the range of microsecond.

10. The method of claim 1, wherein the kind of said specific component of said specimen to be detected depends on an enzyme of said chip.

11. A method for improving a reading resolution of a biosensor, comprising:

providing a specimen on a chip of a biosensor, said chip generating a peak voltage in response to a specific component of said specimen;

selecting a voltage-time discharge curve corresponding to said peak voltage in accordance with a mapping table of peak voltage versus voltage-time discharge curve;

using a voltage V₀ of a sampling time t₀ of said voltage-time discharge curve as a central voltage, and selecting a plurality of respective voltages whose sampling times close to said sampling time t₀, obtaining an average voltage of said central voltage V₀ and said respective voltages to serve as an output voltage corresponding to said sampling time t₀;

obtaining an average voltage corresponding to each said sampling time of said voltage-time discharge curve before the time of discharge ending to serve as an output voltage of each said sampling time based on the former step;

converting said output voltage of each said sampling time of said voltage-time discharge curve to a set of binary digital signals; and

determining a reading of a concentration of said specific component in accordance with the sets of binary digital signals before the time of discharge ending.

12. The method of claim 11, wherein the voltage of each said sampling time of said voltage-time discharge curve is an integer.

13. The method of claim 11, wherein said respective voltages close to said central voltage V₀ includes three voltages V₁, V₂ and V₃ close to said central voltage V₀ in said voltage-time discharge curve.

14. The method of claim 13, wherein said three voltages V₁, V₂ and V₃ includes two voltages prior to said central voltage V₀ and one voltage behind said central voltages V₀ in said voltage-time discharge curve.

15. The method of claim 13, wherein said three voltages includes one voltage prior to said central voltage V₀ and two voltages behind said central voltage V₀ in said voltage-time discharge curve.

16. The method of claim 13, wherein said three voltages are prior to said central voltages V₀ in said voltage-time discharge curve.

17. The method of claim 13, wherein said three voltages are behind said central voltages V₀ in said voltage-time discharge curve.

18. The method of claim 11, wherein the kind of said specific component of said specimen to be detected depends on an enzyme of said chip.

19. The method of claim 11, wherein the sampling interval is in the range of millisecond.

20. The method of claim 11, wherein the sampling time is in the range of microsecond.