Label-free methods for performing assays using a colorimetric resonant optical biosensor
First Claim
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1. A vessel comprising a colorimetric resonant reflectance optical biosensor, wherein the colorimetric resonant reflectance optical biosenseor comprises an internal surface of the vessel, wherein one or more specific binding substances are immobilized at two or more distinct locations on the internal surface of the vessel that comprises a calorimetric resonant reflectance optical biosensor.
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Abstract
The instant invention provides compositions and methods for determining cell interactions that are faster than conventional methods and that require the use of fewer reagents than conventional methods.
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Citations
15 Claims
- 1. A vessel comprising a colorimetric resonant reflectance optical biosensor, wherein the colorimetric resonant reflectance optical biosenseor comprises an internal surface of the vessel, wherein one or more specific binding substances are immobilized at two or more distinct locations on the internal surface of the vessel that comprises a calorimetric resonant reflectance optical biosensor.
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3. A microtiter plate comprising one or more microtiter wells, wherein a bottom surface of the one or more microtiter wells comprises a colorimetric resonant reflectance optical biosensor, wherein one or more specific binding substances are immobilized at two or more distinct locations on the bottom surface of each microtiter well.
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4. A method of detecting binding of one or more types of cells to one or more specific binding substances comprising:
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(a) applying the one or more types of cells to an internal surface of a vessel, wherein the internal surface of the vessel comprises a colorimetric resonant reflectance optical biosensor, wherein one or more specific binding substances are immobilized at two or more distinct locations on the internal surface of the vessel that comprises a calorimetric resonant reflectance optical biosensor;
(b) illuminating the vessel with light;
(c) detecting one or more peak wavelength values (PWV) for each distinct location;
wherein, if the one or more cells have bound to one or more specific binding substances, then the PWV is shifted at the distinct location to which the one or more cells are bound. - View Dependent Claims (5, 6, 7, 8, 9)
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10. A method of detecting binding of one or more cells to one or more specific binding substances comprising:
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(a) immobilizing one or more specific binding substances to two or more distinct locations on an internal surface of a vessel, wherein the internal surface of the vessel comprises a calorimetric resonant reflectance optical biosensor;
(b) illuminating the vessel with light;
(c) detecting one or more peak wavelength values (PWVs) for each distinct location;
(d) applying one or more cells to the internal surface of the vessel;
(e) illuminating the vessel with light;
(f) detecting one or more peak wavelength values (PWVs) for each distinct location;
(g) comparing the PWV'"'"'s of step (c) to the PWV'"'"'s of step (f);
wherein, if the one or more cells have bound to one or more specific binding substances, then the PWV is shifted at the distinct location to which the cells are bound. - View Dependent Claims (11, 12, 13, 14, 15)
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Specification