Preparation of samples and sample evaluation

• US 20040136497A1
• Filed: 10/30/2003
• Published: 07/15/2004
• Est. Priority Date: 10/30/2002
• Status: Abandoned Application

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Turley, Stewart, Fisher, Charles H., Hol, Wim G. J., Moody, Stephen E., Meldrum, Deirdre R.
• Application Number:
  US10/698,234
• Publication Number:
  US 20040136497A1
• Time in Patent Office: Days
• US Class Current: 378/79
• CPC Class Codes: G01N 23/20 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

A capillary tube (10) is provided with ends that are initially open. The capillary tube (10) is preferably constructed from a plastic material that will allow the contents of the tube (10) to be analyzed by x-raying the tube (10). Plural fluid segments (20, 28, 30, 32) are introduced into the capillary tube (10) through one end of the tube (10). Then, the ends of the capillary tube (10) are closed, such as fusing them shut (34, 36) or by providing them with closure caps (40, 42). Different capillary tubes (10) contain different combinations of the fluid segments. The contents of each capillary tube (10) forms a distinct sample. The samples are viewed and evaluated while they are in the sealed capillary tubes (10).

Claims

1. A method of preparing and handling protein samples for x-ray crystallography studies of protein crystals in the samples, comprising:

providing a capillary tube having a sidewall and open ends;

introducing plural fluid segments into the capillary tube;

closing the ends of the capillary tube to seal the tube; and

viewing and evaluating the fluid segments while they are in the sealed tube.

2. The method of claim 1, wherein said capillary tube is a plastic tube.

3. The method of claim 2, wherein the plastic tube is constructed of a plastic that will allow the contents of the tube to be viewed by x-raying the tube.

4. The method claim 1, wherein the fluid segments include a pair of contiguous fluid segments.

5. The method of claim 1, wherein the fluid segments include a pair of axially spaced fluid segments separated by an air gap.

6. The method of claim 1, comprising closing the ends of the capillary tube by heating and pinching the sidewall of the tube at the ends of the tube.

7. The method of claim 1, comprising closing the ends of the capillary tube by use of closure members that engage the ends of the capillary tube and close the ends of the capillary tube.

9. The method of claim 1, comprising introducing the fluid segments in the capillary tube by injecting them in through a first end of the tube.

10. The method of claim 9, comprising subjecting the second end of the tube to a vacuum during injection of the fluid segments into the first end of the tube.

11. The method of claim 10, comprising providing a chuck connected to the vacuum and an end adapted to receive the second end of the tube.

12. The method of claim 11, comprising sealing between the chuck and the end of the tube.

13. The method of claim 1, comprising introducing the fluid segments in the capillary tube by injecting them in through a first end of the tube, and providing plural ejectors, each ejecting a different fluid segment, and moving the first end of the tube into alignment with a first ejector, and operating the injector to introduce a fluid segment of its fluid into the first end of the tube, and then moving the first end of the tube into alignment with a second injector, and operating the second ejector to inject a fluid segment of its fluid into the first end of the tube.

14. The method of claim 13, comprising moving the capillary tube from injector to injector, into positions to receive successive injections from the injectors.

15. The method of claim 1, comprising storing the capillary tube and its contents after the ends of the tube are closed, and periodically evaluating the contents of the tube for crystal formation while in the tube.

16. The method of claim 15, comprising freezing the contents of the tube while it remains in the tube if the evaluation shows a desirable crystal growth in the contents of the tube.

17. The method of claim 16, comprising placing the tube into cold storage while its contents are frozen and storing it in the cold storage.

18. The method of claim 17, comprising removing the tube and its contents from cold storage and making a crystallography evaluation of the contents while it is in the tube and remained cold.

19. The method of claim 18, comprising x-raying the tube and its contents while the contents remain in the tube.

20. The method of claim 1, wherein the fluid segments are segments of different fluids.

21. The method of claim 20, wherein the fluid segments includes a pair of axially spaced fluid segments that are separated by an air gap.

22. The method of claim 21, comprising connecting the first end of the capillary tube with the source of vacuum and using these vacuums to pull moisture out from the samples before closing the ends of the capillary tube to seal the tube.

8. The method of claim 8, comprising using end closures in the form of caps that slip over the ends of the capillary tube.

23. A method of preparing and handling a reagent sample, comprising:

providing a capillary tube having a sidewall and open ends, introducing one or more fluid segments into one end of the capillary tube;

closing the ends of the capillary tube to seal the tube; and

viewing and evaluating the reagent sample while it is in the sealed tube.

24. The method of claim 23, comprising closing the ends of the capillary tube by heating and pinching the sidewall of the tube at the ends of the tube, to cause the sidewalls to fuse and close the ends of the tube.

25. The method of claim 24, comprising closing the ends of the capillary tube by use of closure members that engage the ends of the capillary tube and close the ends of the capillary tube.