Alternative substrates and formats for bead-based array of arrays TM

• US 20040137465A1
• Filed: 08/06/2003
• Published: 07/15/2004
• Est. Priority Date: 02/10/2000
• Status: Abandoned Application

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Kain, Robert, Dickinson, Todd
• Application Number:
  US10/638,173
• Publication Number:
  US 20040137465A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01L 2300/0636 : Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 : Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0822 : Slides
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays. The invention further provides a hybridization chamber for use with a composite array.

Claims

1. A microscope slide composition comprising:

a) a substrate with a surface comprising discrete sites, said sites separated by a distance of less than 50 μ

m, wherein said substrate is formatted to the dimensions of a microscope slide; and

b) a population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactve agent and said second subpopulation comprises a second bioactive agent wherein said microspheres are randomly distributed on said surface.

2. A composition according to claim 1, wherein said sites are separated by a distance of less than 25 μ

m.

3. A composition according to claim 1, wherein said sites are separated by a distance of less than 15 μ

m.

4. A composition according to claim 1, 2 or 3, wherein said sites are separated by a distance of at least about 5 μ

m.

6. The composition according to claim 1 or 5, wherein the distance between centers of a first and second microsphere of said first subpopulation is at least 5 μ

m.

7. The composition according to claim 6, wherein the distance between said first and second microsphere of said first subpopulation is less than about 100 μ

m.

8. A composition according to claim 1 or 5, wherein said substrate further comprises first and second assay locations, wherein said first and second subpopulations are distributed in said first and second assay locations.

9. A composition according to claim 8, wherein the distance between a first and second microsphere of said first subpopulation is less than about 100 μ

m.

10. A composition according to claim 9, wherein the distance between a first and second member of said first subpopulation is less than about 50 μ

m.

11. A composition according to claim 9, wherein the distance between a first and second member of said first subpopulation is less than about 15 μ

m.

12. A composition according to claim 9, 10 or 11, wherein the distance between said first and second member of said first subpopulation is at least about 5 μ

m.

15. An apparatus comprising:

a) a detection instrument; and

b) the composition according to claim 1 or claim 5, wherein said composition is in said instrument.

5. A microscope slide composition comprising:

a) a substrate with a surface comprising discrete sites, wherein said substrate is formatted to the dimensions of a microscope slide;

b) a population of microspheres, comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a bioactive agent and said second subpopulation does not comprise a bioactive agent, wherein said microspheres are randomly distributed on said surface.

13. A composition according to claim 5, wherein said second subpopulation comprises a detectable signal.

14. A composition according to claim 5, wherein said second subpopulation does not comprise a detectable signal.

16. A method for making a microscope slide composition comprising:

a) providing a substrate with a surface comprising wells, wherein said substrate is formatted to the dimensions of a microscope slide;

b) randomly distributing microspheres on said substrate such that individual wells comprise microspheres, wherein said microspheres comprise at least a first and a second subpopulation, wherein said first subpopulation comprises a bioactive agent and said second subpopulation does not comprise a bioactive agent.

17. The method according to claim 16, wherein said first subpopulation further comprises first and second sub-sub-populations, each comprising a first and second bioactive agent, respectively.

18. A method for making a microscope slide composition comprising:

a) providing a substrate with a surface comprising discrete sites, said sites separated by a distance of less than 50 μ

m, wherein said substrate is formatted to the dimensions of a microscope slide; and

b) randomly distributing population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactve agent and said second subpopulation comprises a second bioactive agent.

19. The method according to claim 18 wherein said wells are separated by a distance of less than 25 μ

m.

20. The method according to claim 18, wherein said wells are separated by a distance of less than 15 μ

m.

21. The method according to claim 18, wherein the ratio of said first and said second subpopulation is at least 1:

36.

22. The method according to claim 18, wherein the ratio of said first and said second subpopulation is at least 1:

100.

23. The method according to claim 18, wherein the distance between the centers a first and second microsphere of said first subpopulation is at least 5 μ

m.

24. The method according to claim 18, wherein the distance between the centers of a first and second microsphere of said first subpopulation is at least 15 μ

m.

25. The method according to claim 18, wherein the distance between a first and second microsphere of said first subpopulation is at least 50 μ

m.

26. A method of making microscope slide arrays comprising:

a) providing a substrate comprising at least first and second holes, wherein the diameter of said first and second holes is of a diameter equal to the diameter of a first and second fiber optic bundle, respectively;

b) inserting said first and second fiber optic bundles into said first and second holes, respectively; and

c) cutting said substrate such that the cross section of said first and second fiber bundles is framed by said substrate.