Human myosin-like polypeptide expressed predominantly in heart and muscle
First Claim
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1. An isolated nucleic acid comprising:
- (i) the nucleotide sequence of SEQ ID NO;
1, (ii) the nucleotide sequence of SEQ ID NO;
2, (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO;
2, (iv) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3, (v) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3 with conservative amino acid substitutions;
(vi) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3 with moderately conservative amino acid substitutions, or (vii) a nucleotide sequence that is the complement of the nucleotide sequence of any one of (i)-(vi).
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Abstract
Presented are a novel myosin-like protein particularly expressed in human heart and muscle, isolated nucleic acids encoding the myosin-like protein, compounds and compositions derivable directly or indirectly therefrom, and diagnostic and therapeutic methods for using the same.
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Citations
61 Claims
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1. An isolated nucleic acid comprising:
(i) the nucleotide sequence of SEQ ID NO;
1, (ii) the nucleotide sequence of SEQ ID NO;
2, (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO;
2, (iv) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3, (v) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3 with conservative amino acid substitutions;
(vi) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;
3 with moderately conservative amino acid substitutions, or (vii) a nucleotide sequence that is the complement of the nucleotide sequence of any one of (i)-(vi).- View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 26, 27, 28, 29, 33, 34, 41, 43, 44, 48, 49, 53, 54, 55, 56)
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2. An isolated nucleic acid comprising a nucleotide sequence that hybridizes under high stringency conditions to a probe, the sequence of which probe (i) consists of SEQ ID NO:
- 2, (ii) encodes a polypeptide having the sequence of SEQ ID NO;
3, (iii) encodes a polypeptide having the sequence of SEQ ID NO;
3 with conservative amino acid substitutions, or (iv) is the complement of (i)-(iii), wherein said isolated nucleic acid is nonidentical in sequence to GenBank accession no. AA993492 and is less than 50 kb in length.
- 2, (ii) encodes a polypeptide having the sequence of SEQ ID NO;
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3. An isolated nucleic acid comprising a nucleotide sequence that hybridizes under moderate stringency conditions to a probe, the sequence of which probe (i) consists of SEQ ID NO:
- 2, (ii) encodes a polypeptide having the sequence of SEQ ID NO;
3, (iii) encodes a polypeptide having the sequence of SEQ ID NO;
3 with conservative amino acid substitutions;
or (iv) is the complement of (i)-(iii), wherein said isolated nucleic acid is nonidentical in sequence to GenBank accession no. AA993492 and is less than 50 kb in length.
- 2, (ii) encodes a polypeptide having the sequence of SEQ ID NO;
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4. An isolated nucleic acid comprising a nucleotide sequence that encodes at least 8 contiguous amino acids of SEQ ID NO:
- 3, wherein said isolated nucleic acid is nonidentical in sequence to GenBank accession no. AA993492 and is less than 50 kb in length.
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22. An isolated polypeptide selected from the group consisting of:
- (a) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO;
3;
(b) an isolated polypeptide comprising a fragment of at least 8 amino acids of SEQ ID NO;
3;
(c) an isolated polypeptide according to (a) or (b) in which at least 95% of deviations from the sequence of (a) or (b) are conservative substitutions; and
(d) an isolated polypeptide having at least 65% amino acid sequence identity to the isolated polypeptide of (a) or (b). - View Dependent Claims (23, 24, 25, 30, 35, 36, 37, 38, 39, 40, 42, 45, 46, 47, 50, 51, 52, 57, 58, 59, 60)
- (a) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO;
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31. A method of diagnosing a disease caused by mutation in human hGDMLP-1, comprising:
detecting said mutation in a sample of nucleic acids that derives from a subject suspected to have said disease.
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32. A method of diagnosing or monitoring a disease caused by altered expression of human hGDMLP-1, comprising:
determining the level of expression of human hGDMLP-1 in a sample of nucleic acids or proteins that derives from a subject suspected to have said disease, alterations from a normal level of expression providing diagnostic and/or monitoring information.
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61. A method of screening for agents that modulate the expression of human hGDMLP-1, the method comprising:
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contacting a cell or tissue sample believed to express human hGDMLP-1 with a chemical or biological agent, and then comparing the amount of human hGDMLP-1 expression with that of a control.
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Specification