Progenitor cell populations , expansions thereof, and growth of non-hematopoietic cell types and tissues therefrom
First Claim
1. An enriched progenitor cell population comprising non-hematopoietic progenitor cells extractable from bone marrow, wherein the cell population is substantially devoid of at least one type of hematopoietic progenitor cell.
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Abstract
Described is a method of expanding human progenitor calls by suspension culturing under non-static conditions. The culturing method provides a three-dimensional space for the rapid expansion of desirable progenitors. By this method, a new compartment of multipotential progenitor cells has been identified, which give rise under differentiation conditions to progeny including osteoblasts, chondrocytes, myoblasts, adipocytes, and other non-hematopoietic cell types. Their use in cell and tissue-based engineering is described.
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Citations
26 Claims
- 1. An enriched progenitor cell population comprising non-hematopoietic progenitor cells extractable from bone marrow, wherein the cell population is substantially devoid of at least one type of hematopoietic progenitor cell.
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3. An enriched progenitor cell population according to clam 1, wherein the population is substantially devoid of at least one of the progenitor cell types bearing an MHC determinant selected from HLA-A, HLA-B, WA-C, HLA-DR, HLA-DP and HLA-DQ.
- 5. A method for expanding human non-hematopoietic progenitor cells, the method comprising the step of subjecting a progenitor cell population comprising non-hematopoietic progenitor cells to non-static suspension culturing in a suitable medium for a period of time effective to expand said progenitor cells.
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14. A population of expanded human progenitor cells, characterized in that:
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1) when cultured under conditions appropriate for differentiation, said population is able gives rise to differentiated cell types that include bone cells, fibroblast cells and neuronal cells;
2) the population has been expanded in suspension culture;
3) the progenitor cells within the population have not been selected on the basis of adherence;
4) the population phenotype is SH4 negative, and 5) the population results from culturing in the absence of at least one culture supplement specific for hematopoietic progenitor cell maintenance.
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17. A progenitor cell population as claimed in any one of clams 14, 14 and 15, in combination with one or more factors for inducing the differentiation thereof.
- 18. A method for producing non-hematopoietic cells, comprising the steps of subjecting a progenitor cell population comprising non-hematopoietic progenitor cells to non-static suspension culturing in a suitable, medium for a period of time effective to expand said progenitor cells, and then delivering the expanded progenitor cells or a subpopulation thereof to an environment conducive to the differentiation thereof into the desired-cell type.
- 19. A method of providing cells differentiated from mesenchymal progenitor cells, comprising the steps of subjecting a progenitor cell population comprising mesenchymal progenitor cells to non-static suspension culturing in a suitable medium for a period of time effective to expand said progenitor cells, and then delivering the expanded progenitor cells or a subpopulation thereof to an environment conducive to the differentiation thereof into a mesenchymal cell type.
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26. A cell population representing a new compartment of progenitor cells, said cell population comprising progenitor cells which, under differentiation conditions give rise to a variety of non-hematopoietic cell types including osteoblasts, adipocytes and myoblasts, wherein said cell population results from the non-static suspension culturing of an input population of progenitor cells obtained without prior selection based on cell adherence.
Specification