Apparatus and methods to process substrate surface features
First Claim
Patent Images
1. An apparatus for fluidly separating substrate surface features, comprising:
- at least one separating member that comprises at least one array of apertures disposed through the separating member, which array of apertures comprises a footprint that substantially corresponds to a footprint of at least a portion of an array of wells disposed in a micro-well plate; and
, at least one supporting member structured to support and align at least one substrate so that when the substrate is supported by the supporting member and the supporting member is mated with the separating member, at least one aperture aligns with at least one surface feature of the substrate to fluidly separate the surface feature from at least one other surface feature of the substrate.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides apparatus for fluidly separating substrate surface features. The apparatus include arrays of apertures that correspond to at least a portion of an array of wells disposed in a micro-well plate and/or are structured to fluidly separate multiple surface features disposed on multiple substrates from one another. Related systems, kits, and methods are additionally provided.
191 Citations
106 Claims
-
1. An apparatus for fluidly separating substrate surface features, comprising:
-
at least one separating member that comprises at least one array of apertures disposed through the separating member, which array of apertures comprises a footprint that substantially corresponds to a footprint of at least a portion of an array of wells disposed in a micro-well plate; and
,at least one supporting member structured to support and align at least one substrate so that when the substrate is supported by the supporting member and the supporting member is mated with the separating member, at least one aperture aligns with at least one surface feature of the substrate to fluidly separate the surface feature from at least one other surface feature of the substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65)
-
-
66. An apparatus for fluidly separating substrate surface features, comprising:
-
at least one supporting member structured to support and align two or more substrates; and
,at least one separating member structured to fluidly separate at least two surface features disposed on at least one substrate from one another when the substrates are supported by the supporting member and the supporting member is mated with the separating member. - View Dependent Claims (67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79)
-
-
80. An apparatus for fluidly separating substrate surface features, comprising:
-
at least one separating member that comprises at least one array of apertures disposed through the separating member, which array of apertures comprises a footprint that substantially corresponds to a footprint of at least a portion of an array of wells disposed in a micro-well plate; and
,at least one supporting member structured to support and align two or more substrates so that when the substrates are supported by the supporting member and the supporting member is mated with the separating member, apertures align with surface features of the substrates to fluidly separate at least two surface features disposed on at least one substrate from one another.
-
-
81. A system for processing substrate surface features, the system comprising:
-
at least one apparatus, comprising;
at least one separating member that comprises at least one array of apertures disposed through the separating member, which array of apertures comprises a footprint that substantially corresponds to a footprint of at least a portion of an array of wells disposed in a micro-well plate; and
at least one supporting member structured to support and align at least one substrate so that when the substrate is supported by the supporting member and the supporting member is mated with the separating member, at least one aperture aligns with at least one surface feature of the substrate to fluidly separate the surface feature from at least one other surface feature of the substrate; and
,at least one fluid handling component that dispenses fluids to and/or aspirates fluids from fluidly separated surface features disposed on the substrate, when the substrate is supported by the supporting member and the supporting member is mated with the separating member. - View Dependent Claims (82, 83, 84, 85, 86, 87, 88, 89)
-
-
90. A method of performing array-based assays, the method comprising:
-
providing an apparatus having a footprint that substantially corresponds to a footprint of a micro-well plate and comprising at least one array of one or more probe components disposed on a surface of at least one substrate, wherein the substrate is disposed and aligned in a supporting member that is removably mated with a separating member, which separating member fluidly separates at least one member of the array from at least one other member of the array on the substrate;
contacting selected members of the array with one or more target components; and
,incubating the apparatus for a time that is sufficient to allow interaction, if any, between the target components and the probe components of the selected members, thereby performing the array-based assays. - View Dependent Claims (91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103)
-
-
104. A method of performing array-based assays, the method comprising:
-
providing an apparatus that comprises a plurality of arrays disposed on surfaces of two or more substrates, wherein the substrates are disposed and aligned in a supporting member that is removably mated with a separating member, which separating member fluidly separates at least two members of one or more arrays disposed on at least one substrate from one another;
contacting selected members of the plurality of arrays with one or more target components; and
,incubating the apparatus for a time that is sufficient to allow interaction, if any, between the target components and probe components of the selected members, thereby performing the array-based assays.
-
-
105. A kit to perform array-based assays, comprising:
-
at least one apparatus for fluidly separating substrate surface features that comprises;
at least one separating member that comprises at least one array of apertures disposed through the separating member, which array of apertures comprises a footprint that substantially corresponds to a footprint of at least a portion of an array of wells disposed in a micro-well plate; and
at least one supporting member structured to support and align at least one substrate so that when the substrate is supported by the supporting member and the supporting member is mated with the separating member, at least one aperture aligns with at least one surface feature of the substrate to fluidly separate the surface feature from at least one other surface feature of the substrate; and
,at least one set of instructions that directs use of the apparatus. - View Dependent Claims (106)
-
Specification