Treatment of substrates for immobilizing biomolecules
First Claim
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1. A method of immobilizing biomolecules on a surface of a substrate comprising:
- providing a substrate having a first surface including a functional group for non-covalent attachment to a biomolecule;
contacting at least a portion of the first surface with a reducing agent;
attaching a biomolecule to the functional group.
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Abstract
A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.
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Citations
23 Claims
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1. A method of immobilizing biomolecules on a surface of a substrate comprising:
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providing a substrate having a first surface including a functional group for non-covalent attachment to a biomolecule;
contacting at least a portion of the first surface with a reducing agent;
attaching a biomolecule to the functional group. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of reducing autofluorescence on substrate containing an array of biomolecules comprising:
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providing a substrate having an array of target biomolecules non-covalently attached to at least a first surface of the substrate;
treating at least a portion of the first surface of the substrate with a reducing agent;
hybridizing complementary probe biomolecules to the biomolecules; and
scanning the substrate. - View Dependent Claims (9, 10, 11, 12, 13, 14, 16, 17, 18)
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15. The method of 14 wherein the aqueous solution contains between 0.2% and 0.3% sodium borohydride by volume.
- 19. A method of eliminating autofluorescensce from a substrate coated with a silane comprising treating at least a portion of a first surface of the slide with a reducing agent.
Specification